Moore M A, Tsuda H, Hacker H J, Sato K, Bannasch P, Ito N
First Department of Pathology, Nagoya City University Medical School, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(5):272-8. doi: 10.1007/BF02890253.
Focal proliferative and neoplastic lung lesions induced in Syrian hamsters by dihydroxy-di-n-propylnitrosamine (DHPN) were investigated using a combined histochemical, autoradiographic and electron microscopic approach. Expression of elevated glucose-6-phosphate dehydrogenase (G6PD) and gammaglutamyl-transpeptidase (GGT) activities and levels of immunohistochemically demonstrable glutathione S-transferase placental form (GST-P) were evident in epithelial cells of focal proliferative populations and bronchioloalveolar neoplasms. Binding for the GST-C form, normally only weak, became very pronounced in the stromal elements of DHPN-induced lesions. Increased labelling with tritiated thymidine was associated with increase in morphological atypia within the tumours. Although the enzyme phenotype findings were equivocal the presence of lamellar bodies in some cells of focal proliferative and neoplastic lesions suggested an origin from alveolar type II cells. The present results regarding changed enzyme phenotype in lung lesions suggest important similarities at the biochemical level for the process of neoplasia in the different target organs of DHPN in the hamster and indicate that GST-P may be a useful 'marker' for lung neoplasia.
采用组织化学、放射自显影和电子显微镜相结合的方法，对二羟基二正丙基亚硝胺（DHPN）诱导的叙利亚仓鼠肺部局灶性增生性和肿瘤性病变进行了研究。在局灶性增生性细胞群和细支气管肺泡肿瘤的上皮细胞中，葡萄糖-6-磷酸脱氢酶（G6PD）和γ-谷氨酰转肽酶（GGT）活性升高，免疫组化可检测到的谷胱甘肽S-转移酶胎盘型（GST-P）水平升高。GST-C型的结合通常较弱，但在DHPN诱导的病变的基质成分中变得非常明显。氚标记胸腺嘧啶核苷标记增加与肿瘤内形态学异型性增加相关。尽管酶表型的结果不明确，但局灶性增生性和肿瘤性病变的一些细胞中存在板层小体，提示其起源于II型肺泡细胞。目前关于肺部病变中酶表型改变的结果表明，仓鼠中DHPN不同靶器官肿瘤形成过程在生化水平上存在重要相似性，表明GST-P可能是肺部肿瘤的有用“标志物”。
