Salehi Maedeh, Motallebnejad Mina, Moghadamnia Ali Akbar, Seyemajidi Maryam, Khanghah Simin Noori, Ebrahimpour Alireza, Molania Tahereh
Assistant Professor, Department of Oral and Maxillofacial Medicine, Dental School, Mazandaran University, Sari, Iran.
Professor, Department of Oral and Maxillofacial, Dental Material Research Center, Dental School, Babol, Iran.
J Clin Diagn Res. 2017 Jul;11(7):ZC67-ZC70. doi: 10.7860/JCDR/2017/24887.10249. Epub 2017 Jul 1.
Since oral cancer is one of the causes of mortality, the use of materials or methods that can reduce cancer or prevent its progression has particular importance.
Aim of the study was to evaluate the antitumor effects of Iranian propolis on dysplastic changes of oral mucosa in rats.
This study was performed on 28 Wistar male rats (aged 7-11 weeks, 160±20 g). They were divided into four groups of seven rats. The Group 1 received: 0.5% 7,12-Dimethylbenz[a]anthracene (DMBA), the Group 2: 0.5% DMBA and 100 mg/kg propolis, the Group 3: 0.5% DMBA and 200 mg/kg propolis, and the Group 4: 0.5% DMBA and 400 mg/kg propolis. DMBA in all groups was administered topically (brush) and propolis was injected intraperitoneally. DMBA was brushed twice on the lingual dorsum three times a week for 20 weeks. Propolis injection just every other day and in the days after DMBA was administered for 20 weeks. Rats were sacrificed, and histological examinations were performed on tongue specimen.
Propolis can reduce the degree of dysplasia in doses 100 mg/kg, 200 mg/kg, and 400 mg/kg compared to control (Group 1) (p=0.017, p=0.02, and p=0.002, respectively).
The results of this study showed propolis can dose-dependently prevent DMBA-induced dysplasia of the oral mucosa in animal model.
由于口腔癌是致死原因之一，使用能够减少癌症或预防其进展的材料或方法具有特别重要的意义。
本研究的目的是评估伊朗蜂胶对大鼠口腔黏膜发育异常变化的抗肿瘤作用。
本研究对28只雄性Wistar大鼠（7 - 11周龄，体重160±20克）进行。将它们分为四组，每组七只大鼠。第1组接受：0.5%的7,12 - 二甲基苯并[a]蒽（DMBA），第2组：0.5%的DMBA和100毫克/千克的蜂胶，第3组：0.5%的DMBA和200毫克/千克的蜂胶，第4组：0.5%的DMBA和400毫克/千克的蜂胶。所有组中的DMBA均通过局部（涂抹）给药，蜂胶通过腹腔注射给药。每周三次在舌背涂抹DMBA，共20周。蜂胶每隔一天注射一次，在给予DMBA后的日子里持续注射20周。处死大鼠，并对舌标本进行组织学检查。
与对照组（第1组）相比，100毫克/千克、200毫克/千克和400毫克/千克剂量的蜂胶可降低发育异常程度（p分别为0.017、0.02和0.002）。
本研究结果表明，在动物模型中，蜂胶可剂量依赖性地预防DMBA诱导的口腔黏膜发育异常。
