应用气相色谱-质谱联用（GC-MS）分析来鉴定用不同溶剂制备的伊朗蜂胶的化学成分，并评估其生物活性。
Applying GC-MS analysis to identify chemical composition of Iranian propolis prepared with different solvent and evaluation of its biological activity.
作者信息
Asgharpour Fariba, Moghadamnia Ali Akbar, Kazemi Sohrab, Nouri Hamid Reza, Motallebnejad Mina
机构信息
Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
出版信息
Caspian J Intern Med. 2020;11(2):191-198. doi: 10.22088/cjim.11.2.191.
BACKGROUND
Propolis as a natural product has shown beneficial effects on human health. This study was aimed to investigate the chemical compositions and biological activity of three different extracts of propolis from two distinct geographic areas in Iran.
METHODS
The chemical composition of Iranian propolis extracts that were collected in the Spring of 2016 from two provinces in northern Iran: Ardabil and Polur in Mazandaran Province were measured through gas chromatography-mass spectrometry (GC-MS) methods. In addition, antimicrobial activity and cytotoxicity effect on HN5 and LNCaP cell lines were evaluated. The data were analyzed using one-way ANOVA and p<0.05 was considered as significant.
RESULTS
The GC-MS analysis identified the presence of compounds that belonged to the different groups such as aromatics acids and their related esters, flavonoid and flavonoid derivatives and terpenes. Flavanone was the most dominant compound of flavonoids. The maximum growth inhibition was observed against of ethanolic extract of propolis (p<0.05). Moreover, cytotoxicity showed that ethanolic and dichloromethane extracts had more inhibitory effects on cell lines than the water extract.
CONCLUSION
The results determined that extracts had the highest percentage of flavonoids. Therefore, it is expected that the synergistic effect of the main components of propolis is related to the increase of biological activity of propolis.
背景
蜂胶作为一种天然产物，已显示出对人类健康有益的作用。本研究旨在调查来自伊朗两个不同地理区域的三种不同蜂胶提取物的化学成分和生物活性。
方法
通过气相色谱 - 质谱联用（GC - MS）方法测定了2016年春季从伊朗北部两个省份采集的蜂胶提取物的化学成分：阿尔达比勒省以及马赞德兰省的波卢尔地区。此外，评估了对HN5和LNCaP细胞系的抗菌活性和细胞毒性作用。数据采用单因素方差分析进行分析，p<0.05被认为具有显著性。
结果
GC - MS分析鉴定出了属于不同类别的化合物，如芳香酸及其相关酯类、黄酮类和黄酮类衍生物以及萜类。黄烷酮是黄酮类中最主要的化合物。观察到蜂胶乙醇提取物的生长抑制作用最强（p<0.05）。此外，细胞毒性表明，乙醇提取物和二氯甲烷提取物对细胞系的抑制作用比水提取物更强。
结论
结果表明提取物中黄酮类物质的含量最高。因此，预计蜂胶主要成分的协同作用与蜂胶生物活性的提高有关。
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