膜联蛋白 A2（ANXA2）与非结构蛋白 1 相互作用，促进高致病性 H5N1 禽流感病毒的复制。

Annexin A2 (ANXA2) interacts with nonstructural protein 1 and promotes the replication of highly pathogenic H5N1 avian influenza virus.

机构信息

State Avian Influenza Reference Laboratory, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.

Animal Epidemic Diseases Control and Prevention Center of Liaoning Province, Shenyang, China.

出版信息

BMC Microbiol. 2017 Sep 11;17(1):191. doi: 10.1186/s12866-017-1097-0.

BACKGROUND

Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host.

RESULTS

Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer.

CONCLUSIONS

Our results indicate that ANXA2 interacts with NS1 and ANXA2 expression increases HPAIV replication.

背景

非结构蛋白 1（NS1）是一种多功能蛋白，也是禽流感病毒（AIV）复制和发病机制的关键调节因子。研究表明，NS1 可以与多种宿主蛋白相互作用，从而调节病毒的生命周期。我们之前生成了一种针对 NS1 蛋白的单克隆抗体；在当前的研究中，我们使用该抗体免疫沉淀与 NS1 相互作用的宿主蛋白，以更好地了解 NS1 在病毒和宿主之间通讯中所起的作用。

结果

免疫共沉淀实验鉴定出膜联蛋白 A2（ANXA2）是与 NS1 相互作用的靶分子。共聚焦激光扫描显微镜的结果表明，NS1 在细胞质中与 ANXA2 共定位。ANXA2 的过表达显著增加了 H5N1 亚型高致病性禽流感病毒（HPAIV）的滴度，而 siRNA 介导的 ANXA2 敲低则显著抑制了病毒蛋白的表达，并降低了子代病毒的滴度。