Zhou Ming-Yang, Zhang Xi-Ying, Yang Xiao-Deng, Zhang Yan-Jiao, He Hai-Lun, Ning Daling
Shandong Provincial Key Laboratory of Fine Chemicals, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3996-4001. doi: 10.1099/ijsem.0.002241. Epub 2017 Sep 12.
A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, rod-like, oxidase- and catalase-positive bacterium, designated A2-1, was isolated from soil on Ardley Island, South Shetland Islands, Antarctica. Strain A2-1 grew at 4-22 °C (optimum, 10 °C), at pH 6.0-8.0 (optimum, pH 6.5) and with 0-1.5 % NaCl (optimum, 0.5 %), but could not produce flexirubin-type pigments. 16S rRNA gene sequence analysis showed that the isolates belonged to the genus Flavobacterium. Strain A2-1 had the highest 16S rRNA gene sequence similarity to Flavobacterium cucumis, F. ahnfeltiae and F. cheniae with 95.7, 95.6 and 95.4 %, respectively. The strain A2-1 consisted of a clade with F. cucumis and F. cheniae and simultaneously formed a distinct phyletic lineage in the neighbour-joining phylogenetic tree. Polar lipids of the strain included phosphatidylethanolamine (PE), four unidentified aminolipids and one unidentified lipid. The strain A2-1 contained anteiso-C15 : 0 (20.2 %), iso-C15 : 0 (16.2 %) and C15 : 1 G (11.0 %) as the main fatty acids and the only respiratory quinone was menaquinone MK-6. The genomic DNA G+C content was 34.0 mol%. The polyphasic taxonomic study revealed that the strain A2-1 belongs to a novel species within the genus Flavobacterium and the name Flavobacterium ardleyense sp. nov. is proposed. The type strain is A2-1 (=CCTCC AB 2017157=KCTC 52644).
从南极南设得兰群岛阿德莱岛的土壤中分离出一株革兰氏阴性、需氧、产黄色色素、无鞭毛、不滑动、杆状、氧化酶和过氧化氢酶阳性的细菌，命名为A2-1。菌株A2-1在4-22 °C（最适温度为10 °C）、pH 6.0-8.0（最适pH为6.5）和0-1.5 % NaCl（最适浓度为0.5 %）条件下生长，但不能产生柔韧菌素类色素。16S rRNA基因序列分析表明，该分离株属于黄杆菌属。菌株A2-1与黄瓜黄杆菌、安氏黄杆菌和陈氏黄杆菌的16S rRNA基因序列相似性最高，分别为95.7%、95.6%和95.4%。菌株A2-1与黄瓜黄杆菌和陈氏黄杆菌组成一个进化枝，同时在邻接法系统发育树中形成一个独特的系统发育谱系。该菌株的极性脂质包括磷脂酰乙醇胺（PE）、四种未鉴定的氨基脂质和一种未鉴定的脂质。菌株A2-1以anteiso-C15 : 0（20.2 %）、iso-C15 : 0（16.2 %）和C15 : 1 G（11.0 %）为主要脂肪酸，唯一的呼吸醌是甲基萘醌MK-6。基因组DNA G+C含量为34.0 mol%。多相分类研究表明，菌株A2-1属于黄杆菌属中的一个新物种，因此提出了新种名阿德莱黄杆菌（Flavobacterium ardleyense sp. nov.）。模式菌株为A2-1（=CCTCC AB 2017157=KCTC 52644）。
