Ren Qiaomeng, Yu Min, Li Yuying, Zhang Yan, Shi Xiaochong, Wu Yanhong, Su Ying, Wang Yanan, Wang Xiaolei, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2018 Mar;68(3):795-800. doi: 10.1099/ijsem.0.002586. Epub 2018 Jan 17.
A rod-shaped, Gram-staining-negative, strictly aerobic, non-motile bacterium with no flexirubin-type pigment, designated as W201E, was isolated from an intertidal sandy beach in Antarctica. The organism formed faintly yellow, round colonies on marine agar 2216E. The strain required sea salts for growth and grew optimally in the presence of 2 % (w/v) NaCl at pH 7.0, 20 °C. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain W201E belonged to the genus Flavobacterium and showed the highest sequence similarity to Flavobacterium algicola NBRC 102673 (96.5 %). The major respiratory quinone was menaquinone 6, and the predominant fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C15 : 0 3-OH and summed feature 3 (which comprises C16 : 1ω7c and/or C16 : 1ω6c). The polar lipids of strain W201E comprised one phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The DNA G+C content of strain W201E was 34.1 mol%. On the basis of the polyphasic analyses, this isolate was considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium ovatum sp. nov. is proposed. The type strain is W201E (=KCTC 52693=MCCC 1K03251=CGMCC 1.16053).
从南极洲潮间带沙滩分离出一株杆状、革兰氏染色阴性、严格需氧、无运动性且无类胡萝卜素型色素的细菌，命名为W201E。该菌在海洋琼脂2216E上形成浅黄色圆形菌落。该菌株生长需要海盐，在含有2%（w/v）NaCl、pH 7.0、20°C的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株W201E属于黄杆菌属，与嗜藻黄杆菌NBRC 102673的序列相似性最高（96.5%）。主要呼吸醌为甲基萘醌6，主要脂肪酸为异-C15:1 G、异-C15:0、异-C15:0 3-OH和总和特征3（包括C16:1ω7c和/或C16:1ω6c）。菌株W201E的极性脂质包括一种磷脂酰乙醇胺、两种未鉴定的氨基脂质和三种未鉴定的极性脂质。菌株W201E的DNA G+C含量为34.1 mol%。基于多相分析，该分离株被认为代表黄杆菌属中的一个新物种，为此提出了卵形黄杆菌新种的名称。模式菌株为W201E（=KCTC 52693=MCCC 1K03251=CGMCC 1.16053）。
