单细胞 RNA 测序在肾脏病研究中的应用前景。
The promise of single-cell RNA sequencing for kidney disease investigation.
机构信息
Division of Nephrology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
Division of Nephrology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
出版信息
Kidney Int. 2017 Dec;92(6):1334-1342. doi: 10.1016/j.kint.2017.06.033. Epub 2017 Oct 12.
Abstract
Recent techniques for single-cell RNA sequencing (scRNA-seq) at high throughput are leading to profound new discoveries in biology. The ability to generate vast amounts of transcriptomic data at cellular resolution represents a transformative advance, allowing the identification of novel cell types, states, and dynamics. In this review, we summarize the development of scRNA-seq methodologies and highlight their advantages and drawbacks. We discuss available software tools for analyzing scRNA-Seq data and summarize current computational challenges. Finally, we outline ways in which this powerful technology might be applied to discovery research in kidney development and disease.
摘要
近年来，高通量单细胞 RNA 测序（scRNA-seq）技术正在引领生物学领域的重大新发现。能够以细胞分辨率生成大量转录组数据代表了一项变革性的进展，使人们能够识别新型细胞类型、状态和动态。在这篇综述中，我们总结了 scRNA-seq 方法的发展，并强调了它们的优缺点。我们讨论了用于分析 scRNA-Seq 数据的可用软件工具，并总结了当前的计算挑战。最后，我们概述了这项强大技术如何应用于肾脏发育和疾病的发现研究。
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