单细胞 RNA 测序在肾脏疾病研究中的应用。
Single-cell RNA sequencing for the study of kidney disease.
机构信息
The College of Medical Technology, Shanghai University of Medicine & Health Sciences, 279 Zhouzhu Highway, Pudong New Area, 201318, Shanghai, China.
出版信息
Mol Med. 2023 Jul 3;29(1):85. doi: 10.1186/s10020-023-00693-8.
Abstract
The kidney is an important organ for maintaining normal metabolism and stabilising the internal environment, in which, the heterogeneity of cell types has hindered the progress in understanding the mechanisms underlying kidney disease. In recent years the application of single-cell RNA sequencing (scRNA-seq) in nephrology has developed rapidly. In this review, we summarized the technical platform related to scRNA-seq and the role of this technology in investigating the onset and development of kidney diseases, starting from several common kidney diseases (mainly including lupus nephritis, renal cell carcinoma, diabetic nephropathy and acute kidney injury), and provide a reference for the application of scRNA-seq in the study of kidney disease diagnosis, treatment and prognosis.
摘要
肾脏是维持正常代谢和稳定内环境的重要器官，其中细胞类型的异质性阻碍了对肾脏疾病相关机制的理解。近年来，单细胞 RNA 测序（scRNA-seq）在肾脏病学中的应用发展迅速。在这篇综述中，我们总结了与 scRNA-seq 相关的技术平台以及该技术在研究肾脏疾病发病和发展中的作用，从几种常见的肾脏疾病（主要包括狼疮性肾炎、肾细胞癌、糖尿病肾病和急性肾损伤）开始，并为 scRNA-seq 在肾脏疾病诊断、治疗和预后研究中的应用提供参考。
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