Cattelan Natalia, Jennings-Gee Jamie, Dubey Purnima, Yantorno Osvaldo M, Deora Rajendar
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CONICET, CCT-La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.
Infect Immun. 2017 Nov 17;85(12). doi: 10.1128/IAI.00373-17. Print 2017 Dec.
Pertussis, or whooping cough, caused by the obligate human pathogen is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.
由专性人类病原体引起的百日咳，即百日咳，正在全球范围内卷土重来。对这种病原体的大多数研究是使用实验室适应菌株进行的，这些菌株可能无法代表整个物种。该病原体形成生物膜在发病机制中起重要作用。我们对来自两个具有不同疫苗接种计划的国家的原型实验室菌株和当前流行的分离株的生物膜形成能力进行了并列比较。与参考菌株相比，本文检测的所有菌株都能高水平形成生物膜。生物膜结构分析揭示了国家特异性差异，来自美国的菌株形成的生物膜结构更复杂。在临床分离株中观察到细菌过度聚集以及生物膜促进和抑制因子的相互表达。检测到生物膜形成增加与小鼠鼻腔和气管中上皮细胞粘附增强以及细菌定植水平升高之间存在关联。据我们所知，这项工作首次将细菌中生物膜形成增加与动物模型中的定植优势联系起来。我们提出，当前流行菌株增强的生物膜形成能力有助于它们的持续存在、传播和持续循环。
