Zain Nurfadly, Putra Suhartono Taat, Zein Umar, Hariman Herman
Department of Tropical Medicine, Faculty of Medicine, Muhammadiyah University of North Sumatera, Medan, Indonesia.
Department of Pathobiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
Malays J Med Sci. 2017 Mar;24(2):28-32. doi: 10.21315/mjms2017.24.2.4. Epub 2017 Apr 14.
The apoptosis of microvascular endothelial cells causes plasma leakage in dengue haemorrhagic fever patients. The soluble Fas ligand is a protein with molecular weight of 40 kDa that acts as a mediator of apoptosis. This study aimed to prove whether soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection by comparing the soluble Fas ligand levels in dengue fever (DF) and dengue haemorrhagic fever (DHF) patients early in the course of illness.
This was a prospective study. It included 42 dengue patients (22 DF patients and 20 DHF patients) and 20 healthy people as a control group. The soluble Fas ligand was measured by the enzyme-linked immunosorbent assay (ELISA).
Soluble Fas ligand was increased significantly ( < 0.001) in DHF patients (median = 130.19, IQR = 36.26) compared to DF patients (median = 104.73, IQR = 53.94) and the control group (median = 87.16, IQR = 24.91).
Soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection in the early course of the illness. However, a larger sample size and further objective studies are needed to confirm these findings.
微血管内皮细胞凋亡导致登革出血热患者血浆渗漏。可溶性Fas配体是一种分子量为40 kDa的蛋白质，作为细胞凋亡的介质。本研究旨在通过比较登革热（DF）和登革出血热（DHF）患者病程早期的可溶性Fas配体水平，证明可溶性Fas配体是否可作为预测登革热感染严重程度的潜在标志物。
这是一项前瞻性研究。研究对象包括42例登革热患者（22例DF患者和20例DHF患者）以及20名健康人作为对照组。采用酶联免疫吸附测定（ELISA）法检测可溶性Fas配体。
与DF患者（中位数 = 104.73，四分位间距 = 53.94）和对照组（中位数 = 87.16，四分位间距 = 24.91）相比，DHF患者的可溶性Fas配体显著升高（< 0.001）（中位数 = 130.19，四分位间距 = 36.26）。
可溶性Fas配体可作为预测登革热感染病程早期严重程度的潜在标志物。然而，需要更大样本量和进一步的客观研究来证实这些发现。
