Esposito Alfonso, Pompilio Arianna, Bettua Clotilde, Crocetta Valentina, Giacobazzi Elisabetta, Fiscarelli Ersilia, Jousson Olivier, Di Bonaventura Giovanni
Centre for Integrative Biology, University of TrentoTrento, Italy.
Department of Medical, Oral, and Biotechnological Sciences, Center of Excellence on Aging and Translational Medicine (CeSI-MeT), Università degli Studi "G. d'Annunzio" Chieti-PescaraChieti, Italy.
Front Microbiol. 2017 Aug 28;8:1590. doi: 10.3389/fmicb.2017.01590. eCollection 2017.
has been recognized as an emerging multi-drug resistant opportunistic pathogen in cystic fibrosis (CF) patients. We report a comparative genomic and phenotypic analysis of 91 strains from 10 CF patients over a 12-year period. Draft genome analyses included Multi-Locus Sequence Typing (MLST), Single-Nucleotide Polymorphisms (SNPs), and pangenome characterization. Growth rate, biofilm formation, motility, mutation frequency, virulence, and antibiotic susceptibility were determined and compared with population structure over time. The population consisted of 20 different sequence types (STs), 11 of which are new ones. Pangenome and SNPs data showed that this population is composed of three major phylogenetic lineages. All patients were colonized by multiple STs, although most of them were found in a single patient and showed persistence over years. Only few phenotypes showed some correlation with population phylogenetic structure. Our results show that adaptation to CF lung is associated with consistent genotypic and phenotypic heterogeneity. infecting multiple hosts likely experiences different selection pressures depending on the host environment. The poor genotype-phenotype correlation suggests the existence of complex regulatory mechanisms that need to be explored in order to better design therapeutic strategies.
已被公认为囊性纤维化（CF）患者中一种新兴的多重耐药机会性病原体。我们报告了在12年期间对10名CF患者的91株菌株进行的比较基因组和表型分析。草图基因组分析包括多位点序列分型（MLST）、单核苷酸多态性（SNP）和泛基因组特征分析。测定了生长速率、生物膜形成、运动性、突变频率、毒力和抗生素敏感性，并随时间与群体结构进行比较。该群体由20种不同的序列类型（STs）组成，其中11种是新的。泛基因组和SNP数据表明，该群体由三个主要的系统发育谱系组成。所有患者都被多种STs定植，尽管其中大多数仅在一名患者中发现并持续多年。只有少数表型与群体系统发育结构存在一定相关性。我们的结果表明，对CF肺部的适应与一致的基因型和表型异质性有关。感染多个宿主的病原体可能会根据宿主环境经历不同的选择压力。基因型与表型之间的相关性较差表明存在复杂的调控机制，需要进行探索以更好地设计治疗策略。
