Di Guilmi A M, Bonnet J, Peiβert S, Durmort C, Gallet B, Vernet T, Gisch N, Wong Y-S
Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France.
Chem Commun (Camb). 2017 Sep 21;53(76):10572-10575. doi: 10.1039/c7cc05646j.
Propargyl-choline was efficiently incorporated into teichoic acid (TA) polymers on the surface of Streptococcus pneumoniae. The introduction of a fluorophore by click chemistry enabled sufficient labeling of the pneumococcus, as well as its specific detection when mixed with other bacterial species. The labeling is localized at the septal site, suggesting a similar location of the TA and peptidoglycan (PG) synthetic machineries. This method is a unique opportunity to improve our understanding of the spatial location of pneumococcal TA biosynthesis.
炔丙基胆碱被有效地整合到肺炎链球菌表面的磷壁酸（TA）聚合物中。通过点击化学引入荧光团能够对肺炎球菌进行充分标记，并且在与其他细菌种类混合时能够对其进行特异性检测。标记位于隔膜部位，这表明TA和肽聚糖（PG）合成机制的位置相似。该方法为增进我们对肺炎球菌TA生物合成空间位置的理解提供了一个独特的契机。
