Lopez R, Garcia E, Garcia P, Ronda C, Tomasz A
J Bacteriol. 1982 Sep;151(3):1581-90. doi: 10.1128/jb.151.3.1581-1590.1982.
Choline-containing teichoic acid seems to be essential for the adsorption of bacteriophage Dp-1 to pneumococci. This conclusion is based on the following observations: In contrast to pneumococci grown in choline-containing medium, cells grown in medium containing ethanolamine or other submethylated aminoalcohols instead of choline were found to be resistant to infection by Dp-1. Live choline-grown bacteria and heat- or UV-inactivated cells and purified cell walls prepared from these cells were capable of adsorbing phage Dp-1; ethanolamine-grown pneumococci or cell wall preparations were unable to do so. Adsorption of Dp-1 to choline-containing cell walls was competitively inhibited by phosphorylcholine and by several choline-containing soluble cell surface components, such as the Forssman antigen and the teichoic acid-glycan complexes formed by autolytic cell wall degradation. Cell walls prepared from pneumococci grown in ethanolamine or phosphorylethanolamine were inactive. Electron microscopic studies with pneumococci that had segments of choline-containing cell wall material amid ethanolamine-containing regions indicated that the Dp-1 phage particles adsorbed exclusively to the choline-containing surface areas. We suggest that the choline residues of the pneumococcal teichoic acid are essential components of the Dp-1 phage receptors in this bacterium.
含胆碱的磷壁酸似乎是噬菌体Dp - 1吸附到肺炎球菌上所必需的。这一结论基于以下观察结果：与在含胆碱培养基中生长的肺炎球菌不同，在含乙醇胺或其他亚甲基化氨基醇而非胆碱的培养基中生长的细胞对Dp - 1感染具有抗性。在含胆碱培养基中生长的活细菌以及经加热或紫外线灭活的细胞和从这些细胞制备的纯化细胞壁能够吸附噬菌体Dp - 1；在含乙醇胺培养基中生长的肺炎球菌或细胞壁制剂则不能。Dp - 1对含胆碱细胞壁的吸附受到磷酸胆碱以及几种含胆碱的可溶性细胞表面成分（如福斯曼抗原和由自溶细胞壁降解形成的磷壁酸 - 聚糖复合物）的竞争性抑制。由在乙醇胺或磷酸乙醇胺中生长的肺炎球菌制备的细胞壁无活性。对在含乙醇胺区域中有含胆碱细胞壁材料片段的肺炎球菌进行电子显微镜研究表明，Dp - 1噬菌体颗粒仅吸附到含胆碱的表面积上。我们认为肺炎球菌磷壁酸的胆碱残基是该细菌中Dp - 1噬菌体受体的重要组成部分。
