球形芽孢杆菌1593M杀幼虫基因在集胞藻Anacystis nidulans R2中的表达
Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2.
作者信息
Tandeau de Marsac N, de la Torre F, Szulmajster J
机构信息
Institut Pasteur, Unité de Physiologie Microbienne, Paris, France.
出版信息
Mol Gen Genet. 1987 Sep;209(2):396-8. doi: 10.1007/BF00329671.
PMID:2890082
Abstract
A 3.6 kb HindIII DNA fragment from Bacillus sphaericus 1593M was cloned and expressed in Escherichia coli and B. subtilis using pHV33 as shuttle vector and in the cyanobacterium Anacystis nidulans R2 with pUC303 as shuttle vector. The level of toxin activity of the respective recombinant plasmids pGsp04 and pGsp12 against Culex mosquito larvae was found to be the same in Escherichia coli and in the cyanobacterium.
摘要
来自球形芽孢杆菌1593M的一段3.6 kb HindIII DNA片段被克隆，并使用pHV33作为穿梭载体在大肠杆菌和枯草芽孢杆菌中表达，以及使用pUC303作为穿梭载体在蓝藻集胞藻6803中表达。发现各自的重组质粒pGsp04和pGsp12对库蚊幼虫的毒素活性水平在大肠杆菌和蓝藻中是相同的。
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