杀蚊毒素基因在产气水生弯曲杆菌菌株中的表达。
Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.
作者信息
Yap W H, Thanabalu T, Porter A G
机构信息
Institute of Molecular and Cell Biology, National University of Singapore.
出版信息
Appl Environ Microbiol. 1994 Nov;60(11):4199-202. doi: 10.1128/aem.60.11.4199-4202.1994.
Abstract
A series of plasmids bearing the binary toxin genes of Bacillus sphaericus 2297 or 2317.3, the 100-kDa toxin gene of B. sphaericus SSII-1, or the 130-kDa (cryIVB) toxin gene of Bacillus thuringiensis subsp. israelensis were constructed and introduced into Ancylobacter aquaticus by electroporation. The transformed A. aquaticus cells exhibited significant toxicity towards mosquito larvae, demonstrating a potential use of recombinant A. aquaticus for biological control of mosquitoes.
摘要
构建了一系列携带球形芽孢杆菌2297或2317.3的二元毒素基因、球形芽孢杆菌SSII-1的100 kDa毒素基因或苏云金芽孢杆菌以色列亚种的130 kDa（cryIVB）毒素基因的质粒，并通过电穿孔法将其导入水生弯曲杆菌。转化后的水生弯曲杆菌细胞对蚊虫幼虫表现出显著毒性，表明重组水生弯曲杆菌在蚊虫生物防治方面具有潜在用途。
相似文献
1
Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.杀蚊毒素基因在产气水生弯曲杆菌菌株中的表达。
Appl Environ Microbiol. 1994 Nov;60(11):4199-202. doi: 10.1128/aem.60.11.4199-4202.1994.
2
Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae.球形芽孢杆菌和苏云金芽孢杆菌以色列亚种杀蚊毒素通过重组新月柄杆菌表达，新月柄杆菌是水生昆虫幼虫生物防治的载体。
Appl Environ Microbiol. 1992 Mar;58(3):905-10. doi: 10.1128/aem.58.3.905-910.1992.
3
A plasmid encoding a combination of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus confers toxicity against a broad range of mosquito larvae when expressed in Gram-negative bacteria.一种编码来自苏云金芽孢杆菌以色列亚种和球形芽孢杆菌的杀蚊幼虫基因组合的质粒，当在革兰氏阴性细菌中表达时，对多种蚊虫幼虫具有毒性。
FEMS Microbiol Lett. 2003 Nov 21;228(2):259-63. doi: 10.1016/S0378-1097(03)00780-8.
4
Molecular cloning of the 130-kilodalton mosquitocidal delta-endotoxin gene of Bacillus thuringiensis subsp. israelensis in Bacillus sphaericus.苏云金芽孢杆菌以色列亚种130千道尔顿杀蚊δ-内毒素基因在球形芽孢杆菌中的分子克隆
Appl Environ Microbiol. 1990 Jun;56(6):1710-6. doi: 10.1128/aem.56.6.1710-1716.1990.
5
Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of gram-negative bacteria in mosquito control.在偏生侧丝藻中高效合成杀蚊毒素证明了革兰氏阴性菌在蚊虫控制方面的潜力。
Nat Biotechnol. 1996 Mar;14(3):343-7. doi: 10.1038/nbt0396-343.
6
Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression.球形芽孢杆菌毒素蛋白基因向苏云金芽孢杆菌以色列亚种的转移及其表达。
Appl Environ Microbiol. 1990 Feb;56(2):340-4. doi: 10.1128/aem.56.2.340-344.1990.
7
Cloning and expression of the binary toxin gene from Bacillus sphaericus IAB872 in a crystal-minus Bacillus thuringiensis subsp. israelensis.球形芽孢杆菌IAB872二元毒素基因在无晶体苏云金芽孢杆菌以色列亚种中的克隆与表达
Curr Microbiol. 2001 Jul;43(1):21-5. doi: 10.1007/s002840010254.
8
Evolution of resistance to the Bacillus sphaericus Bin toxin is phenotypically masked by combination with the mosquitocidal proteins of Bacillus thuringiensis subspecies israelensis.对球形芽孢杆菌 Bin 毒素的抗性进化通过与苏云金芽孢杆菌亚种 israelensis 的杀蚊蛋白结合而表现出表型掩盖。
Environ Microbiol. 2010 May;12(5):1154-60. doi: 10.1111/j.1462-2920.2010.02156.x. Epub 2010 Feb 7.
9
[Transgenic bioinsecticides inimical to parasites, but imical to environment].[对寄生虫有害但对环境有害的转基因生物杀虫剂]
Wiad Parazytol. 2003;49(1):11-20.
10
Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.苏云金芽孢杆菌以色列亚种的杀蚊毒素Cyt1Aa和Cry11Aa在偏生噬纤维菌中的共表达
Curr Microbiol. 2007 Jan;54(1):58-62. doi: 10.1007/s00284-006-0352-7. Epub 2006 Dec 6.
引用本文的文献
1
Potential for and Other Bacterial Toxins as Biological Control Agents to Combat Dipteran Pests of Medical and Agronomic Importance.作为生物防治剂的潜力及其它细菌毒素，用于防治医学和农艺重要性双翅目害虫。
Toxins (Basel). 2020 Dec 5;12(12):773. doi: 10.3390/toxins12120773.
2
Ingestibility, digestibility, and engineered biological control potential of Flavobacterium hibernum, isolated from larval mosquito habitats.从幼虫蚊子栖息地中分离出的迟缓爱德华氏菌的可摄入性、可消化性和工程生物控制潜力。
Appl Environ Microbiol. 2014 Feb;80(3):1150-8. doi: 10.1128/AEM.03319-13. Epub 2013 Dec 2.
3
Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.苏云金芽孢杆菌以色列亚种的杀蚊毒素Cyt1Aa和Cry11Aa在偏生噬纤维菌中的共表达
Curr Microbiol. 2007 Jan;54(1):58-62. doi: 10.1007/s00284-006-0352-7. Epub 2006 Dec 6.
4
Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus.苏云金芽孢杆菌杀蚊毒素Cry11Aa在水生细菌偏心柄杆菌中的表达。
Curr Microbiol. 2005 Dec;51(6):430-3. doi: 10.1007/s00284-005-0166-z. Epub 2005 Oct 25.
5
Biocontrol of the sugarcane borer Eldana saccharina by expression of the Bacillus thuringiensis cry1Ac7 and Serratia marcescens chiA genes in sugarcane-associated bacteria.通过在甘蔗相关细菌中表达苏云金芽孢杆菌cry1Ac7基因和粘质沙雷氏菌chiA基因对甘蔗螟虫Eldana saccharina进行生物防治。
Appl Environ Microbiol. 2000 Jul;66(7):2804-10. doi: 10.1128/AEM.66.7.2804-2810.2000.
本文引用的文献
1
Influence of transcriptional and translational control sequences on the expression of foreign genes in Caulobacter crescentus.转录和翻译控制序列对新月柄杆菌中外源基因表达的影响。
J Bacteriol. 1994 May;176(9):2603-10. doi: 10.1128/jb.176.9.2603-2610.1994.
2
Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes.芽孢杆菌的杀蚊毒素及其用于蚊子有效生物防治的基因操作。
Microbiol Rev. 1993 Dec;57(4):838-61. doi: 10.1128/mr.57.4.838-861.1993.
3
Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.携带用于在大肠杆菌中调控克隆基因表达的杂合色氨酸-乳糖启动子的载体。
Gene. 1983 Nov;25(2-3):167-78. doi: 10.1016/0378-1119(83)90222-6.
4
Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid.广宿主范围克隆载体：一种农杆菌Ti质粒的黏粒克隆文库。
Plasmid. 1982 Jul;8(1):45-54. doi: 10.1016/0147-619x(82)90040-3.
5
Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance.广宿主范围质粒RK2中对复制和维持至关重要的区域。
J Bacteriol. 1980 Jan;141(1):213-22. doi: 10.1128/jb.141.1.213-222.1980.
6
Gas-vacuolated strains of Microcyclus aquaticus.水生微环菌的产气菌株。
J Bacteriol. 1971 Oct;108(1):236-40. doi: 10.1128/jb.108.1.236-240.1971.
7
Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297.来自球形芽孢杆菌2362和2297的编码51.4千道尔顿和41.9千道尔顿蛋白质的杀蚊毒素基因的序列分析。
J Bacteriol. 1988 May;170(5):2045-50. doi: 10.1128/jb.170.5.2045-2050.1988.
8
Transcriptional analysis of the major surface array gene of Caulobacter crescentus.新月柄杆菌主要表面阵列基因的转录分析。
J Bacteriol. 1988 Oct;170(10):4706-13. doi: 10.1128/jb.170.10.4706-4713.1988.
9
High efficiency transformation of E. coli by high voltage electroporation.通过高压电穿孔实现大肠杆菌的高效转化。
Nucleic Acids Res. 1988 Jul 11;16(13):6127-45. doi: 10.1093/nar/16.13.6127.
10
Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2.球形芽孢杆菌1593M杀幼虫基因在集胞藻Anacystis nidulans R2中的表达
Mol Gen Genet. 1987 Sep;209(2):396-8. doi: 10.1007/BF00329671.
Zotero 插件