Simon Ádám, Oláh János, Komlósi István, Jávor András, Németh József, Szilvássy Zoltán, Reglődi Dóra, Tamás Andrea, Czeglédi Levente
1 Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen , H-4027 Debrecen, Böszörményi út 138 , Hungary.
2 Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen , H-4032 Debrecen, Nagyerdei körút 98 , Hungary.
Acta Biol Hung. 2017 Sep;68(3):237-247. doi: 10.1556/018.68.2017.3.1.
The list of orexigenic and anorexigenic peptides, those are known to alter feed intake, is continuously growing. However, most of them are studied in mammalian species. We aimed to investigate plasma level and mRNA expression of the pituitary adenylate cyclase-activating polypeptide (PACAP), gene expression of its receptor (PAC1), furthermore the gene expression of galanin (GAL), neuromedin U (NMU), and its two receptors (NMUR1 and NMUR2) in the hypothalamus, proventriculus, and jejunum of hens exposed to 40% calorie restriction. Feed restriction resulted in a 88% increase in mRNA and a 27% increase in peptide level of PACAP in proventriculus measured with qPCR and RIA, respectively. Increases were found in the gene expression of PAC1 (49%) and NMUR1 (63%) in the hypothalamus. Higher expressions of peptide encoding genes (76% for PACAP, 41% for NMU, 301% for NMUR1 and 308% for GAL, P < 0.05) were recorded in the jejunum of hens exposed to restricted nutrition. The results indicate that PACAP level responds to calorie restriction in the proventriculus and jejunum, but not in the hypothalamus and plasma.
已知能改变采食量的促食欲肽和抑食欲肽的种类在不断增加。然而，其中大多数是在哺乳动物物种中进行研究的。我们旨在研究垂体腺苷酸环化酶激活多肽（PACAP）的血浆水平和mRNA表达、其受体（PAC1）的基因表达，以及甘丙肽（GAL）、神经介素U（NMU）及其两种受体（NMUR1和NMUR2）在遭受40%热量限制的母鸡下丘脑、腺胃和空肠中的基因表达。饲料限制分别导致用qPCR和RIA检测时，腺胃中PACAP的mRNA增加88%，肽水平增加27%。下丘脑PAC1（49%）和NMUR1（63%）的基因表达增加。在营养受限的母鸡空肠中，记录到肽编码基因的更高表达（PACAP为76%，NMU为41%，NMUR1为301%，GAL为308%，P<0.05）。结果表明，PACAP水平在腺胃和空肠中对热量限制有反应，但在下丘脑和血浆中无反应。
