Baek Chaeyun, Kim Eunji, Shin Su-Kyoung, Choi Sungmi, Yi Hana
Department of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of Korea.
School of Biosystem and Biomedical Science, Korea University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4045-4049. doi: 10.1099/ijsem.0.002244. Epub 2017 Sep 13.
A Gram-negative, motile, rod-shaped, and aerobic bacterial strain, designated LPB0072, was isolated from a Pacific oyster (Crassostrea gigas). Autotrophic growth with hydrogen gas was not observed. Cells oxidized thiosulfate to sulfate and reduced nitrate to nitrite. The complete genome sequence of strain LPB0072 (CP017476) is 4.94 Mb in length and contains 4459 protein-coding genes, with a G+C content of 61.3 mol%. Analysis of the 16S rRNA gene sequence indicated that strain LPB0072 belongs to the genus Hydrogenophaga, with greatest sequence similarity to the type strain of Hydrogenophaga taeniospiralis (97.5 %). The isoprenoid quinone (Q-8) and the major cellular fatty acids (C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0 and C17 : 1ω6c) identified were concordant with the chemotaxonomic properties of the genus Hydrogenophaga. The average nucleotide identities with closely related species were below the suggested boundary for species delineation, indicating that the isolate is a novel species. Numerous physiological and biochemical features also distinguished the isolate from other known Hydrogenophaga species. Based on the polyphasic data presented in this study, strain LPB0072 should be classified as a novel species in the genus Hydrogenophaga, and the name Hydrogenophaga crassostreae sp. nov. is proposed. The type strain is LPB0072 (=KACC 18705=JCM 31188).
从太平洋牡蛎（太平洋牡蛎）中分离出一株革兰氏阴性、运动、杆状、需氧细菌菌株，命名为LPB0072。未观察到该菌株利用氢气进行自养生长。细胞将硫代硫酸盐氧化为硫酸盐，并将硝酸盐还原为亚硝酸盐。菌株LPB0072（CP017476）的完整基因组序列长度为4.94 Mb，包含4459个蛋白质编码基因，G+C含量为61.3 mol%。16S rRNA基因序列分析表明，菌株LPB0072属于噬氢菌属，与螺旋噬氢菌模式菌株的序列相似性最高（97.5%）。鉴定出的类异戊二烯醌（Q-8）和主要细胞脂肪酸（C16:1ω7c和/或C16:1ω6c、C16:0和C17:1ω6c）与噬氢菌属的化学分类学特性一致。与密切相关物种的平均核苷酸同一性低于物种划分的建议界限，表明该分离株是一个新物种。许多生理和生化特征也将该分离株与其他已知的噬氢菌属物种区分开来。根据本研究提供的多相数据，菌株LPB0072应被归类为噬氢菌属中的一个新物种，并提出了螺旋噬氢菌新种（Hydrogenophaga crassostreae sp. nov.）的名称。模式菌株为LPB0072（=KACC 18705=JCM 31188）。
