Laboratory of Physiology, Department of Translational Medicine, University of Eastern Piedmont, via Solaroli 17, 28100 Novara, Italy.
noiVita s.r.l.s. Spin-Off of University of Eastern Piedmont, via A. Canobio 4/6, 28100 Novara, Italy.
Nutrients. 2017 Sep 13;9(9):1008. doi: 10.3390/nu9091008.
This study compares the absorption characteristics of two iron-based dietary supplements and their biocompatibility to bisglycinate iron, a common chelated iron form. The Caco-2 cell line-a model of human intestinal absorption-and GTL-16 cell line-a model of gastric epithelial cells-were used to perform the experiments; in the first experiments, the kinetics of absorption have been evaluated analyzing the divalent metal transporter 1 (DMT1) expression. Three different iron combinations containing 50 µM iron (named Fisioeme, Sideral and bisglycinate) were used for different stimulation times (1-24 h). After this, the effects of the three iron formulations were assessed in both a short and a long time, in order to understand the extrusion mechanisms. The effects of the three different formulations were also analyzed at the end of stimulation period immediately after iron removal, and after some time in order to clarify whether the mechanisms were irreversibly activated. Findings obtained in this study demonstrate that Fisioeme was able to maintain a significant beneficial effect on cell viability compared to control, to Sideral, and to iron bisglycinate. This observation indicates that Fisioeme formulation is the most suitable for gastric and intestinal epithelial cells.
本研究比较了两种基于铁的膳食补充剂的吸收特性及其与常用的螯合铁形式——双甘氨酸铁的生物相容性。使用 Caco-2 细胞系（一种人肠道吸收模型）和 GTL-16 细胞系（一种胃上皮细胞模型）进行实验；在最初的实验中，通过分析二价金属转运蛋白 1（DMT1）的表达来评估吸收动力学。使用三种含有 50 µM 铁的不同铁组合（分别命名为 Fisioeme、Sideral 和双甘氨酸铁）进行不同的刺激时间（1-24 小时）。之后，为了了解排出机制，在短时间和长时间内评估了三种铁配方的效果。为了澄清这些机制是否被不可逆地激活，还在刺激期结束时立即在铁去除后以及一段时间后分析了三种不同配方的效果。本研究的结果表明，与对照、Sideral 和双甘氨酸铁相比，Fisioeme 能够显著维持对细胞活力的有益影响。这一观察结果表明，Fisioeme 配方最适合胃和肠道上皮细胞。
