Verstijnen C P, Arends J W, Moerkerk P T, Geraedts J P, Sekikawa K, Uitendaal M P, Bosman F T
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(4):191-7. doi: 10.1007/BF02890243.
Two cell lines with different in vitro growth characteristics were established from a single mucinous colonic adenocarcinoma. Epithelial cells of the line 5583-E demonstrated anchorage-dependent growth while those of line 5583-S were anchorage-independent and grew as multicellular floating spheroids. Both cell lines shared common characteristics with respect to the expression of differentiation markers (secretory component, carcinoembryonic antigen), mucins and karyotype (trisomy 12 and 14, marker chromosome) but also showed consistent differences. In nude mice 5583-S cells formed moderately differentiated mucinous adenocarcinomas with high carcinoembryonic antigen and mucin production, whereas 5583-E xenografts were poorly differentiated and almost entirely failed to produce carcinoembryonic antigen and mucins. The plating efficiency of 5583-E cells appeared to be greater and doubling time shorter than those of 5583-S cells. Furthermore, 5583-E cells showed an extra isochromosome, 1q. The cell lines were genotypically and phenotypically stable over a period of 2 years. Our results reemphasize that multiple cell lines with heterogeneous phenotypic and genotypic characteristics can be obtained from a single primary tumor.
从一例黏液性结肠腺癌中建立了两种具有不同体外生长特性的细胞系。5583 - E系的上皮细胞表现出锚定依赖性生长，而5583 - S系的上皮细胞则为锚定非依赖性生长，并形成多细胞漂浮球体。两种细胞系在分化标志物（分泌成分、癌胚抗原）、黏蛋白和核型（12号和14号染色体三体、标记染色体）的表达方面具有共同特征，但也存在一致的差异。在裸鼠中，5583 - S细胞形成了中度分化的黏液性腺癌，癌胚抗原和黏蛋白产量较高，而5583 - E异种移植瘤分化较差，几乎完全不产生癌胚抗原和黏蛋白。5583 - E细胞的接种效率似乎高于5583 - S细胞，倍增时间更短。此外，5583 - E细胞显示出一条额外的等臂染色体1q。这些细胞系在两年时间内基因型和表型均保持稳定。我们的结果再次强调，从单一原发性肿瘤中可以获得具有异质表型和基因型特征的多个细胞系。
