Shimada Takayuki, Nagata Naoyoshi, Okahara Koki, Joya Akane, Hayashida Tsunefusa, Oka Shinichi, Sakurai Toshiyuki, Akiyama Junichi, Uemura Naomi, Gatanaga Hiroyuki
Department of Gastroenterology and Hepatology, National Center for Global Health and Medicine, Tokyo, Japan.
AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan.
PLoS One. 2017 Sep 13;12(9):e0184699. doi: 10.1371/journal.pone.0184699. eCollection 2017.
Detection of human herpesviruses (HHVs) other than cytomegalovirus (CMV) in colonic mucosa of individuals with inflammatory bowel disease (IBD) remains unknown. This study identified eight HHVs in the colonic mucosa of individuals with IBD and compared the results with immunocompetent and human immunodeficiency virus (HIV)-infected individuals.
A total of 89 individuals who had colorectal ulcer on colonoscopy were enrolled: 26 with immunocompetency (n = 26), 41 with IBD, and 22 with HIV infection. We examined the colonic ulcers for the presence of eight HHVs-herpes simplex virus (HSV)-1/2, varicella zoster virus (VZV), CMV, Epstein-Barr virus (EBV), HHV-6, HHV-7, and HHV-8-using mucosal PCR.
The IBD group had positivity rates of 0%, 0%, 0%, 53.7%, 24.4%, 39%, 39%, and 0% for HSV-1, HSV-2, VZV, EBV, CMV, HHV-6, HHV-7, and HHV-8, respectively. The positivity rates of EBV and CMV in colonic mucosa increased significantly in the order of the immunocompetent, IBD, and HIV groups (EBV: 23.1%, 53.7%, 72.7%, P for trend = 0.0005; CMV, 7.7%, 24.4%, 54.5%, P for trend = 0.0003, respectively), but no increase was found in the other HHVs. Median mucosal EBV DNA values in the immunocompetent, IBD, and HIV groups were 0, 76, and 287 copies/μg DNA, respectively (P for trend = 0.002). Corresponding median mucosal CMV DNA values were 0, 0, and 17 copies/μg DNA (P for trend = 0.0001). There was no significant difference in the positivity rates of the eight HHVs between ulcerative colitis and Crohn's disease.
The HHVs of EBV, CMV, HHV-6, and HHV-7, but not of HSV-1, HSV-2, VZV, or HHV-8, were identified in the colonic mucosa of IBD individuals. EBV and CMV in colonic mucosa was correlated with host immune status in increasing order of immunocompetent, IBD, and HIV-infected individuals.
炎症性肠病（IBD）患者结肠黏膜中除巨细胞病毒（CMV）以外的人类疱疹病毒（HHV）的检测情况尚不清楚。本研究在IBD患者的结肠黏膜中鉴定出8种HHV，并将结果与免疫功能正常者和人类免疫缺陷病毒（HIV）感染者进行比较。
共纳入89例结肠镜检查发现结肠溃疡的患者：26例免疫功能正常者（n = 26），41例IBD患者，22例HIV感染者。我们采用黏膜PCR检测结肠溃疡中8种HHV的存在情况，即单纯疱疹病毒（HSV）-1/2、水痘带状疱疹病毒（VZV）、CMV、爱泼斯坦-巴尔病毒（EBV）、HHV-6、HHV-7和HHV-8。
IBD组中HSV-1、HSV-2、VZV、EBV、CMV、HHV-6、HHV-7和HHV-8的阳性率分别为0%、0%、0%、53.7%、24.4%、39%、39%和0%。结肠黏膜中EBV和CMV的阳性率按免疫功能正常组、IBD组和HIV组的顺序显著升高（EBV：23.1%、53.7%、72.7%，趋势P = 0.0005；CMV：7.7%、24.4%、54.5%，趋势P = 0.0003），但其他HHV未发现升高。免疫功能正常组、IBD组和HIV组的黏膜EBV DNA中位数分别为0、76和287拷贝/μg DNA（趋势P = 0.002）。相应的黏膜CMV DNA中位数分别为0、0和17拷贝/μg DNA（趋势P = 0.0001）。溃疡性结肠炎和克罗恩病之间8种HHV的阳性率无显著差异。
在IBD患者的结肠黏膜中鉴定出了EBV、CMV、HHV-6和HHV-7这几种HHV，而HSV-1、HSV-2、VZV或HHV-8未被鉴定出。结肠黏膜中的EBV和CMV与宿主免疫状态相关，按免疫功能正常者、IBD患者和HIV感染者的顺序递增。
