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与浆果相关的酵母挥发物改变果蝇的行为。

berry related yeast volatiles modify behaviour of flies.

作者信息

Mozūraitis Raimondas, Aleknavičius Dominykas, Vepštaitė-Monstavičė Iglė, Stanevičienė Ramunė, Emami Seyedeh Noushin, Apšegaitė Violeta, Radžiutė Sandra, Blažytė-Čereškienė Laima, Servienė Elena, Būda Vincas

机构信息

Laboratory of Chemical and Behavioural Ecology, Institute of Ecology, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania.

Laboratory of Genetics, Institute of Botany, Nature Research Centre, Akademijos 2, LT-08412 Vilnius, Lithuania.

出版信息

J Adv Res. 2019 Aug 5;21:71-77. doi: 10.1016/j.jare.2019.08.001. eCollection 2020 Jan.

Abstract

Olfactory cues have a large impact on insect behaviour and fitness consequently showing potential in pest management. Yeast released volatiles are used by insects as olfactory cues for finding feeding and oviposition sites. The yeast strain SB-16-15 was isolated from spontaneous fermentation of berries and identified as . Thirty-nine volatiles were sampled from the headspace of yeasts by solid phase micro extraction and identified by gas chromatography and mass spectrometry techniques. Ten of those volatiles elicited antennal responses of flies, one of the most serious pest of berries. In the two-choice experiments, flies preferred the mixture composed of nine synthetic compounds analogous to electroanntenographic active volatiles released by the yeasts compare to the solvent control. Female flies were significantly attracted to the mixture at the concentration 0.1 µL mL and showed no preference to the mixture at the concentration 1 µL mL versus control while males reacted positively to the synthetic blend at the concentration 1 µL mL. Herein, for the first time, behaviour modifying effect of berry related yeast volatiles was shown suggesting these semiochemicals have potential in use for monitoring flies.

摘要

嗅觉线索对昆虫行为和适应性有很大影响,因此在害虫管理中显示出潜力。昆虫利用酵母释放的挥发性物质作为寻找取食和产卵地点的嗅觉线索。酵母菌株SB - 16 - 15是从浆果的自然发酵中分离出来的,并被鉴定为……通过固相微萃取从酵母的顶空中采集了39种挥发性物质,并通过气相色谱和质谱技术进行了鉴定。其中10种挥发性物质引起了果蝇(浆果最严重的害虫之一)的触角反应。在双选实验中,与溶剂对照相比,果蝇更喜欢由九种类似于酵母释放的电触角图活性挥发性物质的合成化合物组成的混合物。雌蝇在浓度为0.1µL/mL时被该混合物显著吸引,而在浓度为1µL/mL时与对照相比对该混合物无偏好,而雄蝇在浓度为1µL/mL时对合成混合物有积极反应。在此首次表明了与浆果相关的酵母挥发性物质的行为改变作用,表明这些信息化学物质在监测果蝇方面具有应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/753e/7015468/e6b7135a7d74/ga1.jpg

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