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不同木糖利用酵母发酵能力的比较评估。

Comparative assessment of fermentative capacity of different xylose-consuming yeasts.

机构信息

Brazilian Agricultural Research Corporation, EMBRAPA Agroenergia, Parque Estação Biológica, PqEB-W3 Norte Final-s/nº, Brasília, DF, CEP 70.770-901, Brazil.

Graduate Program on Molecular Biology, Department of Cellular Biology, University of Brasília, Campus Darcy Ribeiro, Brasília, DF, Brazil.

出版信息

Microb Cell Fact. 2017 Sep 13;16(1):153. doi: 10.1186/s12934-017-0766-x.

DOI:10.1186/s12934-017-0766-x
PMID:28903764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5598047/
Abstract

BACKGROUND

Understanding the effects of oxygen levels on yeast xylose metabolism would benefit ethanol production. In this work, xylose fermentative capacity of Scheffersomyces stipitis, Spathaspora passalidarum, Spathaspora arborariae and Candida tenuis was systematically compared under aerobic, oxygen-limited and anaerobic conditions.

RESULTS

Fermentative performances of the four yeasts were greatly influenced by oxygen availability. S. stipitis and S. passalidarum showed the highest ethanol yields (above 0.44 g g) under oxygen limitation. However, S. passalidarum produced 1.5 times more ethanol than S. stipitis under anaerobiosis. While C. tenuis showed the lowest xylose consumption rate and incapacity to produce ethanol, S. arborariae showed an intermediate fermentative performance among the yeasts. NAD(P)H xylose reductase (XR) activity in crude cell extracts correlated with xylose consumption rates and ethanol production.

CONCLUSIONS

Overall, the present work demonstrates that the availability of oxygen influences the production of ethanol by yeasts and indicates that the NADH-dependent XR activity is a limiting step on the xylose metabolism. S. stipitis and S. passalidarum have the greatest potential for ethanol production from xylose. Both yeasts showed similar ethanol yields near theoretical under oxygen-limited condition. Besides that, S. passalidarum showed the best xylose consumption and ethanol production under anaerobiosis.

摘要

背景

了解氧气水平对酵母木糖代谢的影响将有利于乙醇的生产。在这项工作中，系统比较了酿酒酵母、节杆菌、树状节杆菌和脆壁克鲁维酵母在好氧、缺氧和厌氧条件下的木糖发酵能力。

结果

四种酵母的发酵性能受氧气供应的影响很大。在缺氧条件下，S. stipitis 和 S. passalidarum 的乙醇产量最高（高于 0.44 g g）。然而，S. passalidarum 在厌氧条件下产生的乙醇比 S. stipitis 多 1.5 倍。虽然 C. tenuis 的木糖消耗率和产乙醇能力最低，但 S. arborariae 的发酵性能在四种酵母中处于中间水平。粗细胞提取物中的 NAD(P)H 木糖还原酶（XR）活性与木糖消耗率和乙醇产量相关。

结论

总之，本研究表明氧气的可用性影响酵母乙醇的生产，并表明 NADH 依赖的 XR 活性是木糖代谢的限制步骤。酿酒酵母和节杆菌具有从木糖生产乙醇的最大潜力。在缺氧条件下，两种酵母的乙醇产率均接近理论值。此外，S. passalidarum 在厌氧条件下表现出最好的木糖消耗和乙醇生产能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/744b/5598047/f7468be329ef/12934_2017_766_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/744b/5598047/6aaed88a2194/12934_2017_766_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/744b/5598047/f7468be329ef/12934_2017_766_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/744b/5598047/6aaed88a2194/12934_2017_766_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/744b/5598047/f7468be329ef/12934_2017_766_Fig2_HTML.jpg

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