Harikrishna N, Mahalakshmi S, Kiran Kumar K, Reddy Gopal
Government Degree College, Siddipet, Telangana India.
Department of Microbiology, Osmania University, Hyderabad, 500007 India.
Indian J Microbiol. 2017 Sep;57(3):339-343. doi: 10.1007/s12088-017-0664-2. Epub 2017 Jul 25.
Fish processing industries generate large quantities of fish scales as processing waste, if not treated leading to environmental pollution. Fish scales are hard to degrade, hence cause difficulty in waste management. In this context present study was made to utilize fish scales as substrate for the production of alkaline protease by GVC11 and subsequently amino acid rich aqua hydrolyzate. GVC11 efficiently utilized five types of fish scales as substrates and produced maximum alkaline protease using (28,150 U/mL) followed by (23,320 U/mL) at 48 h and (17,146 U/mL) (18,917 U/mL) (12,430 U/mL) at 72 h. The HPLC analysis of protein hydrolyzate obtained after fermentation was enriched in essential amino acids, leucine, isoleucine, phenylalanine, lysine and non-essential amino acids, tyrosine, arginine and cysteine which can be used as animal feed supplement and organic fertilizer.
鱼类加工行业会产生大量鱼鳞作为加工废弃物，若不加以处理会导致环境污染。鱼鳞难以降解，因此给废物管理带来困难。在此背景下，开展了本研究，以鱼鳞为底物，利用GVC11生产碱性蛋白酶，随后生产富含氨基酸的水产水解产物。GVC11能有效利用五种鱼鳞作为底物，在48小时时产生的碱性蛋白酶最多（28,150 U/mL），其次是（23,320 U/mL），在72小时时分别为（17,146 U/mL）（18,917 U/mL）（12,430 U/mL）。发酵后获得的蛋白质水解产物的HPLC分析表明，其富含必需氨基酸亮氨酸、异亮氨酸、苯丙氨酸、赖氨酸以及非必需氨基酸酪氨酸、精氨酸和半胱氨酸，这些可作为动物饲料补充剂和有机肥料。
