Nagamalli Harikrishna, Sitaraman Mahalakshmi, Kandalai Kiran Kumar, Mudhole Gopal Reddy
Government Degree College, Siddipet, Telangana, India.
Department of Microbiology, Osmania University, Hyderabad, 500007, India.
3 Biotech. 2017 Jul;7(3):185. doi: 10.1007/s13205-017-0801-y. Epub 2017 Jun 29.
Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The resultant protein hydrolyzate after fermentation was found to have essential amino acids such as leucine, phenyl alanine, isoleucine, lysine, valine, methionine, arginine in considerable quantities and minute concentrations of cysteine. The protein hydrolyzate was also found to have good antioxidant activity.
以鸡蛋壳和蛋膜为底物，由高海拔芽孢杆菌GVC11生产碱性蛋白酶。添加麦芽糖作为额外碳源可使酶产量提高13%。添加蛋白胨和酵母提取物等有机氮源分别使酶产量提高了9%和5%，而无机氮源没有任何积极作用。发酵后的蛋白质水解产物含有大量必需氨基酸，如亮氨酸、苯丙氨酸、异亮氨酸、赖氨酸、缬氨酸、蛋氨酸、精氨酸，以及微量的半胱氨酸。还发现该蛋白质水解产物具有良好的抗氧化活性。
