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将 MERS 刺突蛋白 ELISA 纳入算法以确定 MERS-CoV 感染的血清学证据。

Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.

机构信息

ORISE Fellowship Training Program, Oak Ridge, Tennessee.

Respiratory Viruses Branch, Div. of Viral Diseases, Natl. Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta, Georgia.

出版信息

J Med Virol. 2018 Feb;90(2):367-371. doi: 10.1002/jmv.24948. Epub 2017 Sep 26.

DOI:10.1002/jmv.24948
PMID:28906003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6158782/
Abstract

The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations. In the current study, we describe development and validation of an indirect MERS-CoV S ELISA to be used as part of our serological determination for evidence of previous exposure to the virus.

摘要

疾病预防控制中心（CDC）的算法，用于检测潜在感染中东呼吸综合征冠状病毒（MERS-CoV）的患者血清中针对该病毒的抗体的存在情况，包括针对重组核衣壳（N）蛋白的间接 ELISA 筛选和通过感染单层细胞的免疫荧光染色和/或微量中和滴定的确认。其他国际小组包括使用刺突（S）蛋白的间接 ELISA 检测，作为其血清学测定的一部分。在本研究中，我们描述了间接 MERS-CoV S ELISA 的开发和验证，该方法将用于我们的血清学测定，以确定先前是否接触过该病毒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2194/7166690/db947c1cf7db/JMV-90-367-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2194/7166690/aaee2a37e91d/JMV-90-367-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2194/7166690/db947c1cf7db/JMV-90-367-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2194/7166690/aaee2a37e91d/JMV-90-367-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2194/7166690/db947c1cf7db/JMV-90-367-g002.jpg

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J Microbiol. 2017 Mar;55(3):172-182. doi: 10.1007/s12275-017-7026-y. Epub 2017 Feb 28.
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