Iriuchijima T, Prasad C, Wilber J F, Jayaraman A, Rao J K, Robertson H J, Rogers D J
Department of Medicine, Louisiana State University Medical Center, New Orleans 70112.
Life Sci. 1987 Nov 30;41(22):2419-28. doi: 10.1016/0024-3205(87)90667-9.
Levels of thyrotropin-releasing hormone (TRH) - and cyclo(His-Pro) (CHP)-like immunoreactivities and the activity of enzyme Pyroglutamate aminopeptidase (PAPase) were measured in cerebrospinal fluid (CSF) of over 100 normal adults (NA) and infants, and adult patients with various neurologic and neuropsychiatric disorders (NNDA). Levels of TRH and CHP in CSF of over 70% of the NA group were below 50 and 500 pg/ml respectively. The TRH- and CHP-like immunoreactivities in the remainder of the 30% of NA specimens exhibiting higher peptide concentrations were enzymatically and chromatographically characterized and were found to behave like authentic peptides. The levels of both of these peptides were significantly elevated in the CSF of most of the NNDA patients. An elevation in the CSF level of CHP was significantly correlated with the level of TRH, but not PAPase. Results from this study suggest that CSF elevation of TRH level may be due to a nonspecific response to stress that may be associated with hospitalization, myelogram procedure, and/or the neurologic and neuropsychiatric diseases for which the patients were admitted.
在100多名正常成年人（NA）、婴儿以及患有各种神经和神经精神疾病（NNDA）的成年患者的脑脊液（CSF）中，检测了促甲状腺激素释放激素（TRH）和环（组氨酸-脯氨酸）（CHP）样免疫反应性以及焦谷氨酸氨基肽酶（PAPase）的活性。超过70%的NA组CSF中TRH和CHP水平分别低于50和500 pg/ml。在30%的NA样本中，其余肽浓度较高的样本中，对TRH和CHP样免疫反应性进行了酶学和色谱分析，发现其行为类似于真实肽。在大多数NNDA患者的CSF中，这两种肽的水平均显著升高。CSF中CHP水平的升高与TRH水平显著相关，但与PAPase无关。这项研究的结果表明，CSF中TRH水平的升高可能是由于对应激的非特异性反应，这可能与住院、脊髓造影检查和/或患者所患的神经和神经精神疾病有关。
