Jayachandran Muthuvel, Garovic Vesna D, Mielke Michelle M, Bailey Kent R, Lahr Brian D, Miller Virginia M
Departments of Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, United States of America.
PLoS One. 2017 Sep 14;12(9):e0183159. doi: 10.1371/journal.pone.0183159. eCollection 2017.
Mechanisms and interactions among intravascular cells contributing to development of subclinical atherosclerosis are poorly understood. In women, both menopausal status and pregnancy history influence progression of atherosclerosis. This study examined activation and interactions among blood elements with subclinical atherosclerosis in menopausal women with known pregnancy histories.
Carotid intima-media thickness (CIMT), as a marker of subclinical atherosclerosis, was measured using B-mode ultrasound in age- and parity-matched women [40 with and 40 without a history of preeclampsia] 35 years after the index pregnancy. Interactions among intravascular cells (38 parameters) were measured by flow cytometry in venous blood. Data analysis was by principal component which retained 7 independent dimensions accounting for 63% of the variability among 38 parameters.
CIMT was significantly greater in women with a history of preeclampsia (P = 0.004). Platelet aggregation and platelet interactions with granulocytes and monocytes positively associated with CIMT in postmenopausal women independent of their pregnancy history (ρ = 0.258, P< 0.05). However, the association of the number of platelets, platelet activation and monocyte-platelet interactions with CIMT differed significantly depending upon pregnancy history (test for interaction, P<0.001).
Interactions among actived intravascular cells and their association with subclinical atherosclerosis differ in women depending upon their pregnancy histories.
血管内细胞之间促进亚临床动脉粥样硬化发展的机制及相互作用尚未完全明确。在女性中，绝经状态和妊娠史都会影响动脉粥样硬化的进展。本研究调查了有已知妊娠史的绝经后女性血液成分的激活情况及其与亚临床动脉粥样硬化的相互作用。
在妊娠指标出现35年后，采用B型超声测量年龄和产次匹配的女性[40例有先兆子痫病史，40例无先兆子痫病史]的颈动脉内膜中层厚度（CIMT），作为亚临床动脉粥样硬化的标志物。通过流式细胞术检测静脉血中血管内细胞之间的相互作用（38个参数）。数据分析采用主成分分析法，保留7个独立维度，占38个参数变异性的63%。
有先兆子痫病史的女性CIMT显著更高（P = 0.004）。绝经后女性中，血小板聚集以及血小板与粒细胞和单核细胞的相互作用与CIMT呈正相关，且与她们的妊娠史无关（ρ = 0.258，P<0.05）。然而，血小板数量、血小板激活以及单核细胞 - 血小板相互作用与CIMT的关联因妊娠史不同而有显著差异（交互作用检验，P<0.001）。
激活的血管内细胞之间的相互作用及其与亚临床动脉粥样硬化的关联在女性中因妊娠史不同而有所差异。
