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人胎盘钠钾ATP酶α亚基：cDNA克隆、组织表达、DNA多态性及染色体定位

Human placental Na+,K+-ATPase alpha subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization.

作者信息

Chehab F F, Kan Y W, Law M L, Hartz J, Kao F T, Blostein R

机构信息

Howard Hughes Medical Institute University of California, San Francisco 94143.

出版信息

Proc Natl Acad Sci U S A. 1987 Nov;84(22):7901-5. doi: 10.1073/pnas.84.22.7901.

DOI:10.1073/pnas.84.22.7901
PMID:2891135
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299443/
Abstract

A 2.2-kilobase clone comprising a major portion of the coding sequence of the Na+,K+-ATPase alpha subunit was cloned from human placenta and its sequence was identical to that encoding the alpha subunit of human kidney and HeLa cells. Transfer blot analysis of the mRNA products of the Na+,K+-ATPase gene from various human tissues and cell lines revealed only one band (approximately 4.7 kilobases) under low and high stringency washing conditions. The levels of expression in the tissues were intestine greater than placenta greater than liver greater than pancreas, and in the cell lines the levels were human erythroleukemia greater than butyrate-induced colon greater than colon greater than brain greater than HeLa cells. mRNA was undetectable in reticulocytes, consistent with our failure to detect positive clones in a size-selected (greater than 2 kilobases) lambda gt11 reticulocyte cDNA library. DNA analysis revealed a polymorphic EcoRI band and chromosome localization by flow sorting and in situ hybridization showed that the alpha subunit is on the short arm (band p11-p13) of chromosome 1.

摘要

从人胎盘中克隆出一个包含钠钾ATP酶α亚基编码序列大部分的2.2千碱基克隆，其序列与编码人肾和HeLa细胞α亚基的序列相同。对来自各种人体组织和细胞系的钠钾ATP酶基因的mRNA产物进行转移印迹分析，发现在低严谨度和高严谨度洗涤条件下均只有一条带（约4.7千碱基）。在组织中的表达水平为：肠道＞胎盘＞肝脏＞胰腺；在细胞系中的表达水平为：人红白血病细胞＞丁酸盐诱导的结肠细胞＞结肠细胞＞脑＞HeLa细胞。在网织红细胞中未检测到mRNA，这与我们在大小选择（大于2千碱基）的λgt11网织红细胞cDNA文库中未能检测到阳性克隆一致。DNA分析揭示了一个多态性的EcoRI带，通过流式分选和原位杂交进行的染色体定位显示α亚基位于1号染色体的短臂（带p11 - p13）上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/9e46d118bf1b/pnas00337-0112-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/4d5ff0c6b983/pnas00337-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/2597c4d28489/pnas00337-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/6fadd2f8dae1/pnas00337-0112-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/1460bc13f81e/pnas00337-0112-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/9e46d118bf1b/pnas00337-0112-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/4d5ff0c6b983/pnas00337-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/2597c4d28489/pnas00337-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/6fadd2f8dae1/pnas00337-0112-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/1460bc13f81e/pnas00337-0112-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d79/299443/9e46d118bf1b/pnas00337-0112-e.jpg

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High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11.高分辨率染色体分选和DNA斑点印迹分析将麦克尔憩室综合征定位于11号染色体。
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