Harrison G S, Drabkin H A, Kao F T, Hartz J, Hart I M, Chu E H, Wu B J, Morimoto R I
Somat Cell Mol Genet. 1987 Mar;13(2):119-30. doi: 10.1007/BF01534692.
The HSP70 family of heat-shock proteins constitutes the major proteins synthesized in response to elevated temperatures and other forms of stress. In eukaryotes members of the HSP70 family also include a protein similar if not identical to bovine brain uncoating ATPase and glucose-regulated proteins. An intriguing relation has been established between expression of heat-shock proteins and transformation in mammalian cells. Elevated levels of HSP70 are found in some transformed cell lines, and viral and cellular gene products that are capable of transforming cells in vitro can also stimulate transcription of HSP70 genes. To determine the organization of this complex multigene family in the human genome, we used complementary approaches: Southern analysis and protein gels of Chinese hamster-human somatic cell hybrids, and in situ hybridization to human chromosomes. We demonstrate that functional genes encoding HSP70 proteins map to human chromosomes 6, 14, 21, and at least one other chromosome.
热休克蛋白HSP70家族是在温度升高及其他形式的应激反应中合成的主要蛋白质。在真核生物中，HSP70家族成员还包括一种与牛脑脱衣ATP酶和葡萄糖调节蛋白相似（即便不是完全相同）的蛋白质。热休克蛋白的表达与哺乳动物细胞的转化之间已建立了一种有趣的关系。在一些转化细胞系中发现HSP70水平升高，并且能够在体外转化细胞的病毒和细胞基因产物也能刺激HSP70基因的转录。为了确定这个复杂的多基因家族在人类基因组中的组织方式，我们采用了互补方法：对中国仓鼠-人类体细胞杂种进行Southern分析和蛋白质凝胶分析，以及对人类染色体进行原位杂交。我们证明，编码HSP70蛋白的功能基因定位于人类染色体6、14、21以及至少一条其他染色体上。
