Yang Runqiang, Guo Yuanxin, Wang Shufang, Gu Zhenxin
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China; College of Food and Drug, Anhui Science and Technology University, Fengyang, Anhui, PR China.
J Food Drug Anal. 2015 Jun;23(2):287-293. doi: 10.1016/j.jfda.2014.07.004. Epub 2014 Nov 6.
Gamma-aminobutyric acid (GABA), a nonproteinous amino acid with some benefits on human health, is synthesized by GABA-shunt and the polyamine degradation pathway in plants. The regulation of Ca and aminoguanidine on GABA accumulation in germinating soybean (Glycine max L.) under hypoxia-NaCl stress was investigated in this study. Exogenous Ca increased GABA content significantly by enhancing glutamate decarboxylase gene expression and its activity. Addition of ethylene glycol tetra-acetic acid into the culture solution reduced GABA content greatly due to the inhibition of glutamate decarboxylase activity. Aminoguanidine reduced over 85% of diamine oxidase activity, and 33.28% and 36.35% of GABA content in cotyledon and embryo, respectively. Under hypoxia-NaCl stress, the polyamine degradation pathway contributed 31.61-39.43% of the GABA formation in germinating soybean.
γ-氨基丁酸（GABA）是一种对人体健康有益的非蛋白质氨基酸，在植物中通过GABA分流途径和多胺降解途径合成。本研究探讨了钙和氨基胍对缺氧 - 氯化钠胁迫下萌发大豆（Glycine max L.）中GABA积累的调控作用。外源钙通过增强谷氨酸脱羧酶基因表达及其活性显著提高了GABA含量。向培养液中添加乙二醇四乙酸由于抑制了谷氨酸脱羧酶活性而大大降低了GABA含量。氨基胍降低了超过85%的二胺氧化酶活性，子叶和胚中的GABA含量分别降低了33.28%和36.35%。在缺氧 - 氯化钠胁迫下，多胺降解途径在萌发大豆的GABA形成中占31.61 - （此处原文可能有误，推测应为31.61%-39.43%）39.43%。
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