Medicinal Natural Products Research Laboratory (MNPRL), Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (E) 400 019, Mumbai, India.
J Food Drug Anal. 2017 Apr;25(2):425-429. doi: 10.1016/j.jfda.2016.06.006. Epub 2016 Jul 25.
This study was undertaken to isolate and quantify aristolochic acid in Aristolochia indica stem and Apama siliquosa root. Aristolochic acid is an important biomarker component present in the Aristolochiaceae family. The isolation method involved simple solvent extraction, precipitation and further purification, using recrystallization. The structure of the compound was confirmed using infrared spectroscopy, mass spectrometry and nuclear magnetic resonance. A specific and rapid high-performance thin layer chromatography (HPTLC) method was developed for analysis of aristolochic acid. The method involved separation on the silica gel 60 F plates using the single solvent system of n-hexane: chloroform: methanol. The method showed good linear relationship in the range 0.4-2.0 μg/spot with r = 0.998. The limit of detection and limit of quantification were 62.841 ng/spot and 209.47 ng/spot, respectively. The proposed validated HPTLC method was found to be an easy to use, accurate and convenient method that could be successfully used for standardization and quality assessment of herbal material as well as formulations containing different species of the Aristolochiaceae family.
本研究旨在从马兜铃科植物关木通的茎和防己科植物蝙蝠葛的根中分离和定量检测马兜铃酸。马兜铃酸是马兜铃科植物中的一种重要生物标志物成分。采用简单的溶剂提取、沉淀和进一步的纯化方法，包括重结晶来分离该化合物。使用红外光谱、质谱和核磁共振对化合物的结构进行了确认。建立了一种用于分析马兜铃酸的快速、特异的高效薄层色谱（HPTLC）方法。该方法在 0.4-2.0μg/斑点范围内表现出良好的线性关系，r=0.998。检测限和定量限分别为 62.841ng/斑点和 209.47ng/斑点。该经过验证的 HPTLC 方法简单易用，准确方便，可成功用于草药材料的标准化和质量评估以及含有不同马兜铃科植物的制剂。
