Sannikova Natalia, Lewis Andrew R, Bennet Andrew J
Simon Fraser University, Burnaby, BC, Canada.
Simon Fraser University, Burnaby, BC, Canada.
Methods Enzymol. 2017;596:547-571. doi: 10.1016/bs.mie.2017.06.034. Epub 2017 Jul 25.
Nuclear magnetic spectroscopic (NMR) methods are discussed for the measurement of heavy atom (C, O, N) and secondary deuterium kinetic isotope effects. The discussion focuses primarily on the NMR methods that enable the measurement of quantitative spectra and not on methods to make labeled substrates. Two main techniques are considered: single-point determinations on natural abundance material and the continuous monitoring of isotopically enriched materials. The second method is described in more detail, and we include a discussion of the current state of instrumentation and computer programs for data acquisition and analysis.
本文讨论了用于测量重原子（碳、氧、氮）和二级氘动力学同位素效应的核磁共振光谱（NMR）方法。讨论主要集中在能够测量定量光谱的NMR方法上，而不是制备标记底物的方法。文中考虑了两种主要技术：对天然丰度材料进行单点测定以及对同位素富集材料进行连续监测。对第二种方法进行了更详细的描述，并且我们还讨论了用于数据采集和分析的仪器设备及计算机程序的当前状态。
