Institute of Microbiology, CAS, Centre Algatech, Laboratory of Cell Cycles of Algae, CZ-379 81 Třeboň, Czech Republic.
Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic.
Biotechnol Adv. 2018 May-Jun;36(3):784-797. doi: 10.1016/j.biotechadv.2018.01.010. Epub 2018 Jan 19.
Stable isotopes are used in wide fields of application from natural tracers in biology, geology and archeology through studies of metabolic fluxes to their application as tracers in quantitative proteomics and structural biology. We review the use of stable isotopes of biogenic elements (H, C, N, O, S, Mg, Se) with the emphasis on hydrogen and its heavy isotope deuterium. We will discuss the limitations of enriching various compounds in stable isotopes when produced in living organisms. Finally, we overview methods for measuring stable isotopes, focusing on methods for detection in single cells in situ and their exploitation in modern biotechnologies.
稳定同位素在广泛的应用领域中被使用，从生物学、地质学和考古学中的天然示踪剂，到代谢通量的研究，再到定量蛋白质组学和结构生物学中的示踪剂。我们回顾了生物元素（H、C、N、O、S、Mg、Se）的稳定同位素的应用，重点是氢及其重同位素氘。我们将讨论在生物体中产生时，各种化合物稳定同位素富集的局限性。最后，我们综述了测量稳定同位素的方法，重点是原位单细胞检测方法及其在现代生物技术中的应用。
