Zhang Xuefeng, Hu Linkun, Du Mingzhan, Wei Xuedong, Zhang Jun, Hui Yu, Chen Cheng, Li Gang, Hou Jianquan
Department of Urology, First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215031, China.
Department of Pathology, First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215031, China.
Pathol Oncol Res. 2018 Oct;24(4):885-890. doi: 10.1007/s12253-017-0302-7. Epub 2017 Sep 14.
Eukaryotic elongation factor 2 (eEF2), a key regulator of protein synthesis, is involved in the progression of several types of cancer. This first study was to investigate the relationships between eEF2 protein and prostate cancer (PCa). Immunohistochemical staining was used to verify eEF2 protein in a set of 97 formalin-fixed, paraffin-embedded primary PCa tissues. Expression of eEF2 protein in positive cells was characterized by cytoplasmic staining. Correlations with clinicopathological factors were evaluated by Chi-square or Fisher's exact probability tests. eEF2 protein was found in 74 out of 97 (76.29%) patients. eEF2-positive had higher PSA and Gleason score than negative in all patients. In addition, the positive expression of eEF2 protein was significantly associated with PSA and Gleason score (P = 0.007 and 0.002). However, no significant correlations occurred between expression of eEF2 protein and TNM stage (P = 0.292). In those eEF2 protein-positive patients, we have found staining intensity of eEF2 protein was not only associated with PSA and Gleason score, but also associated with TNM stage (P = 0, 0.014 and 0.001, respectively). To conclude, our study indicates that expression of eEF2 protein is a potential biomarker for evaluating PCa.
真核生物延伸因子2（eEF2）是蛋白质合成的关键调节因子，参与多种癌症的进展。本首次研究旨在探讨eEF2蛋白与前列腺癌（PCa）之间的关系。采用免疫组织化学染色法在一组97例福尔马林固定、石蜡包埋的原发性PCa组织中验证eEF2蛋白。eEF2蛋白在阳性细胞中的表达以细胞质染色为特征。通过卡方检验或Fisher精确概率检验评估与临床病理因素的相关性。97例患者中有74例（76.29%）检测到eEF2蛋白。在所有患者中，eEF2阳性患者的PSA和Gleason评分均高于阴性患者。此外，eEF2蛋白的阳性表达与PSA和Gleason评分显著相关（P = 0.007和0.002）。然而，eEF2蛋白表达与TNM分期之间无显著相关性（P = 0.292）。在那些eEF2蛋白阳性的患者中，我们发现eEF2蛋白的染色强度不仅与PSA和Gleason评分相关，还与TNM分期相关（分别为P = 0、0.014和0.001）。总之，我们的研究表明eEF2蛋白表达是评估PCa的潜在生物标志物。
