HIV 患者血清中免疫调节剂 Semaphorin 4D（CD100）水平较低。

Low levels of the immunoregulator Semaphorin 4D (CD100) in sera of HIV patients.

机构信息

Allergy and Clinical Immunology Unit, Bnei-Zion Medical Center, Haifa, Israel.

Department of Medicine B, Allergy, Clinical Immunology and AIDS Center, Kaplan Medical Center, Rehovot, Israel.

出版信息

Clin Immunol. 2018 Jun;191:88-93. doi: 10.1016/j.clim.2017.09.010. Epub 2017 Sep 14.

DOI:10.1016/j.clim.2017.09.010

PMID:28917721

Abstract

INTRODUCTION

Semaphorin-4D (CD100), generated by CD4/CD8 T-cells and its receptor on B cells - CD72, play a role in immune regulation. Both have soluble forms - sCD100/sCD72.

METHODS

sCD100 and sCD72 levels were determined by ELISA (MyBioSource, USA).

RESULTS

28 chronic HIV patients and 50 matched healthy volunteers participated in our study. Before treatment, CD4 T-cells counts were 267 ± 216 cells/mcl and viral load (VL) was 586,675 ± 1897,431 copies/ml. Two years following HAART, CD4 T-cells counts rose to 475 ± 264 cells/mcl and VL dropped to 2050 ± 10,539 copies/ml. CD8 T-cells counts were stable. sCD72 levels prior (4.13 ± 2.03 ng/ml) and following HAART (3.53 ± 2.01 ng/ml) were similar to control levels (4.51 ± 2.66 ng/ml). sCD100 levels before (40.47 ± 31.4 ng/ml) and following HAART (37.68 ± 29.44 ng/ml) were significantly lower compared to controls (99.67 ± 36.72 ng/ml) despite the significant increase in CD4 T-cells counts.

CONCLUSIONS

The permanent low levels of the immunoregulator sCD100 suggest a role for CD100 in the immune dysfunction and T cells exhaustion of HIV.

摘要

简介

信号素-4D（CD100）由 CD4/CD8 T 细胞产生，其受体位于 B 细胞上——CD72，在免疫调节中发挥作用。两者都有可溶性形式——sCD100/sCD72。

方法

通过 ELISA（美国 MyBioSource）测定 sCD100 和 sCD72 水平。

结果

28 名慢性 HIV 患者和 50 名匹配的健康志愿者参加了我们的研究。治疗前，CD4 T 细胞计数为 267±216 个/µl，病毒载量（VL）为 586675±1897431 拷贝/ml。接受 HAART 治疗两年后，CD4 T 细胞计数上升至 475±264 个/µl，VL 降至 2050±10539 拷贝/ml。CD8 T 细胞计数保持稳定。HAART 治疗前后 sCD72 水平（分别为 4.13±2.03ng/ml 和 3.53±2.01ng/ml）与对照组（4.51±2.66ng/ml）相似。尽管 CD4 T 细胞计数显著增加，但 sCD100 水平在治疗前（40.47±31.4ng/ml）和治疗后（37.68±29.44ng/ml）均显著低于对照组（99.67±36.72ng/ml）。