Katz B Z, Niederman J C, Olson B A, Miller G
Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510.
J Infect Dis. 1988 Feb;157(2):299-308. doi: 10.1093/infdis/157.2.299.
DNA restriction fragment length polymorphisms of Epstein-Barr virus (EBV) DNA were used as a molecular epidemiological tool to study multiple isolates of virus from the same and different individuals. We studied 35 EBV isolates: 19 from seven immunocompromised children and 16 from seven college students with mononucleosis. Analysis of the fragment length polymorphisms in this collection of isolates permitted several conclusions. Sites of polymorphism were most often encountered in regions with repetitive DNA. Epidemiologically unrelated patients harbored viruses that could be readily distinguished; by contrast, two infants and their mothers harbored similar viruses. Isolates from different sites in the same patient were similar. Variations between different clinical isolates of EBV mimic those found between different laboratory strains of the virus. Fragment length polymorphisms thus provide a useful marker for studying transmission and pathogenesis of EBV infections.
爱泼斯坦-巴尔病毒（EBV）DNA的限制性片段长度多态性被用作一种分子流行病学工具，以研究来自同一和不同个体的多个病毒分离株。我们研究了35株EBV分离株：19株来自7名免疫功能低下的儿童，16株来自7名患单核细胞增多症的大学生。对这组分离株的片段长度多态性分析得出了几个结论。多态性位点最常出现在具有重复DNA的区域。流行病学上不相关的患者携带的病毒很容易区分；相比之下，两名婴儿及其母亲携带相似的病毒。同一患者不同部位的分离株相似。EBV不同临床分离株之间的变异类似于该病毒不同实验室菌株之间的变异。因此，片段长度多态性为研究EBV感染的传播和发病机制提供了一个有用的标记。
