Miller G, Katz B Z, Niederman J C
Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut.
Yale J Biol Med. 1987 Jul-Aug;60(4):307-19.
We have applied two different recombinant DNA techniques to the study of the epidemiology of Epstein-Barr virus infections. In the first application, cloned subfragments of viral DNA were used as probes to detect EBV DNA in a variety of lymphoproliferative disorders and in lymphoid cell lines. Patients who are epidemiologically unrelated harbor EBV genotypes which can readily be distinguished from each other. Patients who are epidemiologically related (such as mothers and infants) have similar EBV genotypes. Some patients, especially those who are immunocompromised, are infected with two distinct genotypes. In the second application, we have examined the immune response to specific EBV antigens expressed from small cloned viral DNA subfragments. We have identified a group of patients with presumed chronic EBV infection who selectively fail to recognize one subcomponent of the EB nuclear antigen complex.
我们应用了两种不同的重组DNA技术来研究爱泼斯坦-巴尔病毒感染的流行病学。在首次应用中，病毒DNA的克隆亚片段被用作探针，以检测各种淋巴增殖性疾病和淋巴样细胞系中的EBV DNA。在流行病学上无关联的患者携带的EBV基因型很容易相互区分。在流行病学上相关的患者（如母亲和婴儿）具有相似的EBV基因型。一些患者，尤其是免疫功能低下的患者，感染了两种不同的基因型。在第二次应用中，我们检测了对从小的克隆病毒DNA亚片段表达的特定EBV抗原的免疫反应。我们已经确定了一组推测为慢性EBV感染的患者，他们选择性地无法识别EB核抗原复合物的一个亚成分。
