马尾藻岩藻聚糖硫酸酯及其寡糖的结构、酶解转化与抗癌活性
Structure, enzymatic transformation, anticancer activity of fucoidan and sulphated fucooligosaccharides from Sargassum horneri.
机构信息
Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia.
Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia.
出版信息
Carbohydr Polym. 2017 Nov 1;175:654-660. doi: 10.1016/j.carbpol.2017.08.043. Epub 2017 Aug 12.
Structure and anticancer activity of fucoidan from Sargassum horneri and from products of its enzymatic transformation were investigated. A gene that encodes fucoidanase ffa1 in the marine bacteria F. algae was identified, cloned and the protein (FFA1) was produced in Escherichia coli. The mass of the gene product FFA1 is 111kDa. Sequence analysis has revealed that fucoidanase FFA1 belongs to the GH107 (CAZy) family. Recombinant fucoidanase FFA1 was used to produce fucooligosaccharides. Structure of 5 sulphated oligosaccharides with polymerization degree 4-10 was established by NMR-spectroscopy. The fucoidan extracted from S. horneri is almost pure fucan. The main chain of the fucoidan is established to consist mostly of the repeating →3-α-l-Fucp(2SO)-1→4-α-l-Fucp(2,3SO)-1→ fragment, with insertions of →3-α-l-Fucp(2,4SO)-1→ fragment. Unsulphated side chains with the α-l-Fucp-1→2-α-l-Fucp-1→ structure connect to the main one at the C4 of monosaccharide residue.
研究了来自马尾藻的褐藻胶和其酶转化产物的结构和抗癌活性。从海洋细菌 F. algae 中鉴定、克隆了编码褐藻胶酶 ffa1 的基因，并在大肠杆菌中表达了该蛋白（FFA1）。FFA1 基因产物的分子量为 111kDa。序列分析表明，褐藻胶酶 FFA1 属于 GH107（CAZY）家族。重组褐藻胶酶 FFA1 用于生产岩藻低聚糖。通过 NMR 光谱法确定了聚合度为 4-10 的 5 个硫酸化寡糖的结构。从马尾藻中提取的褐藻胶几乎是纯的岩藻聚糖。褐藻胶的主链主要由重复的→3-α-l-Fucp(2SO)-1→4-α-l-Fucp(2,3SO)-1→片段组成，插入→3-α-l-Fucp(2,4SO)-1→片段。带有α-l-Fucp-1→2-α-l-Fucp-1→结构的未硫酸化侧链在单糖残基的 C4 处与主链相连。
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