Yan Jun, Su Peisen, Wei Zhaoran, Nevo Eviatar, Kong Lingrang
College of Information Science and Engineering, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Plant Mol Biol. 2017 Oct;95(3):227-242. doi: 10.1007/s11103-017-0637-1. Epub 2017 Sep 16.
In this study we systematically identified and classified PKs in Triticum aestivum, Triticum urartu and Aegilops tauschii. Domain distribution and exon-intron structure analyses of PKs were performed, and we found conserved exon-intron structures within the exon phases in the kinase domain. Collinearity events were determined, and we identified various T. aestivum PKs from polyploidizations and tandem duplication events. Global expression pattern analysis of T. aestivum PKs revealed that some PKs might participate in the signaling pathways of stress response and developmental processes. QRT-PCR of 15 selected PKs were performed under drought treatment and with infection of Fusarium graminearum to validate the prediction of microarray. The protein kinase (PK) gene superfamily is one of the largest families in plants and participates in various plant processes, including growth, development, and stress response. To better understand wheat PKs, we conducted genome-wide identification, classification, evolutionary analysis and expression profiles of wheat and Ae. tauschii PKs. We identified 3269, 1213 and 1448 typical PK genes in T. aestivum, T. urartu and Ae. tauschii, respectively, and classified them into major groups and subfamilies. Domain distributions and gene structures were analyzed and visualized. Some conserved intron-exon structures within the conserved kinase domain were found in T. aestivum, T. urartu and Ae. tauschii, as well as the primitive land plants Selaginella moellendorffii and Physcomitrella patens, revealing the important roles and conserved evolutionary history of these PKs. We analyzed the collinearity events of T. aestivum PKs and identified PKs from polyploidizations and tandem duplication events. Global expression pattern analysis of T. aestivum PKs revealed tissue-specific and stress-specific expression profiles, hinting that some wheat PKs may regulate abiotic and biotic stress response signaling pathways. QRT-PCR of 15 selected PKs were performed under drought treatment and with infection of F. graminearum to validate the prediction of microarray. Our results will provide the foundational information for further studies on the molecular functions of wheat PKs.
在本研究中，我们系统地鉴定并分类了普通小麦、乌拉尔图小麦和节节麦中的蛋白激酶（PKs）。对PKs进行了结构域分布和外显子 - 内含子结构分析，我们发现在激酶结构域的外显子阶段存在保守的外显子 - 内含子结构。确定了共线性事件，我们从多倍体化和串联重复事件中鉴定出了各种普通小麦PKs。普通小麦PKs的全局表达模式分析表明，一些PKs可能参与应激反应和发育过程的信号通路。在干旱处理和禾谷镰刀菌感染条件下，对15个选定的PKs进行了定量逆转录聚合酶链反应（QRT-PCR），以验证微阵列的预测结果。蛋白激酶（PK）基因超家族是植物中最大的家族之一，参与各种植物过程，包括生长、发育和应激反应。为了更好地了解小麦PKs，我们对小麦和节节麦的PKs进行了全基因组鉴定、分类、进化分析和表达谱分析。我们分别在普通小麦、乌拉尔图小麦和节节麦中鉴定出3269、1213和1448个典型的PK基因，并将它们分为主要类别和亚家族。分析并可视化了结构域分布和基因结构。在普通小麦、乌拉尔图小麦和节节麦以及原始陆生植物小立碗藓和小立碗藓中，在保守的激酶结构域内发现了一些保守的内含子 - 外显子结构，揭示了这些PKs的重要作用和保守进化历史。我们分析了普通小麦PKs的共线性事件，并从多倍体化和串联重复事件中鉴定出PKs。普通小麦PKs的全局表达模式分析揭示了组织特异性和应激特异性表达谱，这表明一些小麦PKs可能调节非生物和生物应激反应信号通路。在干旱处理和禾谷镰刀菌感染条件下，对15个选定的PKs进行了QRT-PCR，以验证微阵列的预测结果。我们的结果将为进一步研究小麦PKs的分子功能提供基础信息。
