Clinical Pathology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Physical Medicine, Rheumatology and Rehabilitation department, Faculty of Medicine, Suez Canal University, Ismailia, 41111, Egypt.
Clin Rheumatol. 2017 Dec;36(12):2719-2726. doi: 10.1007/s10067-017-3818-z. Epub 2017 Sep 16.
B cells are essential players in the pathogenic mechanisms of systemic lupus erythematosus (SLE). Although CD5+ B cells have been considered to play a paradoxical role in preventing, rather than inducing autoimmunity, there is no consensus agreement about the proportions of CD5+ B cells population in SLE patients. So, the aim of the present study was to assess blood concentration of CD5+ B cells in patients with SLE and to evaluate their relationship with disease activity and organ damage. We recruited 100 SLE patients and 100 healthy control subjects. Based on SLE disease activity index (SLEDAI), patients were divided into two groups: active SLE (n = 50) and inactive SLE (n = 50). SLE was active when SLEDAI was ≥ 4. The expression of CD5+ B cells was evaluated using flow cytometry to measure the proportions and absolute numbers of the cells. The proportions of CD5+ B cells of total lymphocytes were significantly lower in SLE patients versus controls (4.1 ± 3.9 vs 10.8 ± 5.2%, P = <0.001). CD5+ B cells were significantly decreased in active SLE patients (3.1 ± 2.7%) in comparison to inactive patients (5.2 ± 3.7%) (P = 0.013). CD5+ B cells correlated positively with C3 (r = 0.328, P = 0.020) and C4 (r = 0.355, P = 0.011). CD5+ B cells were significantly decreased in SLE patients compared to healthy controls and they were significantly decreased in active SLE patients in comparison to inactive ones.
B 细胞是系统性红斑狼疮（SLE）发病机制中的重要参与者。虽然 CD5+ B 细胞被认为在预防而不是诱导自身免疫中发挥矛盾作用，但对于 SLE 患者中 CD5+ B 细胞群体的比例尚无共识。因此，本研究旨在评估 SLE 患者血液中 CD5+ B 细胞的浓度，并评估其与疾病活动度和器官损伤的关系。我们招募了 100 例 SLE 患者和 100 例健康对照者。根据 SLE 疾病活动指数（SLEDAI），患者分为两组：活动期 SLE（n=50）和非活动期 SLE（n=50）。当 SLEDAI≥4 时，SLE 为活动期。使用流式细胞术评估 CD5+ B 细胞的表达，以测量细胞的比例和绝对数。与对照组相比，SLE 患者的总淋巴细胞中 CD5+ B 细胞的比例显著降低（4.1±3.9% vs 10.8±5.2%，P<0.001）。与非活动期患者（5.2±3.7%）相比，活动期患者（3.1±2.7%）CD5+ B 细胞明显减少（P=0.013）。CD5+ B 细胞与 C3（r=0.328，P=0.020）和 C4（r=0.355，P=0.011）呈正相关。与健康对照组相比，SLE 患者的 CD5+ B 细胞明显减少，与非活动期患者相比，活动期患者的 CD5+ B 细胞明显减少。
