College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
College of Life and Health Sciences, Northeastern University, Shenyang, Liaoning, 110819, China.
Environ Sci Pollut Res Int. 2017 Nov;24(32):25000-25009. doi: 10.1007/s11356-017-0179-8. Epub 2017 Sep 17.
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography. The 5240 peptide was stable at 100 °C for 20 min and remained active throughout a wide pH range (4-10). The antagonistic activity was not affected by protease K and trypsin. The purified 5240 peptide exhibited a broad inhibitory spectrum against various plant pathogenic fungi and was identified as iturin A (C-C). Moreover, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry indicated the presence of fengycin A (C-C), fengycin B (C-C), and surfactin (C-C) isoforms in supernatants from strain W10. These results suggest that B. amyloliquefaciens W10 has significant potential as a biocontrol agent.
从番茄根际中分离到的芽孢杆菌 W10 产生的抗真菌代谢产物进行了研究。通过分析其 16S rDNA 和 gyrB 基因部分序列，将菌株 W10 鉴定为解淀粉芽孢杆菌。PCR 分析显示存在编码丰原素、表面活性剂和伊曲菌素的 fenB、sfp 和 ituD 基因。通过硫酸铵沉淀和 Superdex 200 凝胶过滤层析从该菌株中分离出一种新型的小抗真菌肽，命名为 5240。该肽在 100°C 下稳定 20 分钟，在广泛的 pH 范围内（4-10）保持活性。拮抗活性不受蛋白酶 K 和胰蛋白酶的影响。纯化的 5240 肽对各种植物病原真菌表现出广谱抑制活性，被鉴定为伊曲菌素 A（C-C）。此外，基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱表明，W10 菌株上清液中存在丰原素 A（C-C）、丰原素 B（C-C）和表面活性剂（C-C）异构体。这些结果表明，解淀粉芽孢杆菌 W10 具有作为生物防治剂的巨大潜力。
