Zammuto Vincenzo, Rizzo Maria Giovanna, De Pasquale Claudia, Ferlazzo Guido, Caccamo Maria Teresa, Magazù Salvatore, Guglielmino Salvatore Pietro Paolo, Gugliandolo Concetta
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy.
Research Centre for Extreme Environments and Extremophiles, Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy.
Microorganisms. 2023 Jul 20;11(7):1842. doi: 10.3390/microorganisms11071842.
We report the ability of the crude biosurfactant (BS B3-15), produced by the marine, thermotolerant B3-15, to hinder the adhesion and biofilm formation of ATCC 27853 and ATCC 29213 to polystyrene and human cells. First, we attempted to increase the BS yield, optimizing the culture conditions, and evaluated the surface-active properties of cell-free supernatants. Under phosphate deprivation (0.06 mM) and 5% saccharose, the yield of BS (1.5 g/L) increased by 37%, which could be explained by the earlier (12 h) increase in AA expression compared to the non-optimized condition (48 h). Without exerting any anti-bacterial activity, BS (300 µg/mL) prevented the adhesion of and to polystyrene (47% and 36%, respectively) and disrupted the preformed biofilms, being more efficient against (47%) than (26%). When added to human cells, the BS reduced the adhesion of and (10× and 100,000× CFU/mL, respectively) without altering the epithelial cells' viability. As it is not cytotoxic, BS B3-15 could be useful to prevent or remove bacterial biofilms in several medical and non-medical applications.
我们报告了海洋耐热菌株B3-15产生的粗生物表面活性剂（BS B3-15）抑制ATCC 27853和ATCC 29213对聚苯乙烯及人类细胞的黏附与生物膜形成的能力。首先，我们尝试通过优化培养条件提高生物表面活性剂的产量，并评估无细胞上清液的表面活性特性。在磷酸盐缺乏（0.06 mM）和5%蔗糖条件下，生物表面活性剂的产量（1.5 g/L）提高了37%，这可以解释为与未优化条件（48小时）相比，AA表达提前（12小时）增加。在不发挥任何抗菌活性的情况下，生物表面活性剂（300 µg/mL）可防止ATCC 27853和ATCC 29213对聚苯乙烯的黏附（分别为47%和36%），并破坏预先形成的生物膜，对ATCC 27853（47%）的效果比对ATCC 29213（26%）更显著。当添加到人类细胞中时，生物表面活性剂可降低ATCC 27853和ATCC 29213的黏附（分别为10倍和100,000倍CFU/mL），且不改变上皮细胞的活力。由于生物表面活性剂B3-15无细胞毒性，它在多种医学和非医学应用中对于预防或去除细菌生物膜可能有用。
