College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Pestic Biochem Physiol. 2018 Sep;150:78-82. doi: 10.1016/j.pestbp.2018.07.006. Epub 2018 Jul 18.
Bacillus sp. 6256 is a good biocontrol agent against Botrytis cinerea which caused tomato gray mold disease. Strain 6256 was identified as B. amyloliquefaciens by analysis of its partial gyrB gene sequence. To identify and characterize the antimycotic peptides from the culture broth of the bacterium, the antimicrobial substances produced by B. amyloliquefaciens 6256 were isolated by ammonium sulfate precipitation, Superdex 200 gel filtration chromatography and DEAE anion exchange chromatography. The purified compound was designated as P657. The biological activity of P657 was stable at as high as 100 °C for 20 min and in pH value ranged from 5 to 10. The antimycotic compound was resistant to trypsin and proteinase K, and could completely inhibit spore germination of Botrytis cinerea in vitro. MALDI-TOF-MS analysis results showed the presence of fengycins A (C-C) and fengycins B (C-C) isoforms in P657.
芽孢杆菌 6256 是一种防治番茄灰霉病的生防菌，对灰葡萄孢有较好的抑制作用。通过对其部分 gyrB 基因序列分析，将菌株 6256 鉴定为解淀粉芽孢杆菌。为了鉴定和分析该细菌发酵液中的抗真菌肽，采用硫酸铵沉淀、Superdex 200 凝胶过滤层析和 DEAE 阴离子交换层析等方法对解淀粉芽孢杆菌 6256 产生的抑菌物质进行分离，得到的纯化化合物命名为 P657。P657 的生物活性在高达 100°C 下 20 分钟内稳定，在 pH 值 5 到 10 的范围内稳定。该抗真菌化合物对胰蛋白酶和蛋白酶 K 具有抗性，能完全抑制灰葡萄孢孢子的体外萌发。MALDI-TOF-MS 分析结果表明，P657 中存在丰余菌素 A（C-C）和丰余菌素 B（C-C）异构体。
