Tuna Bilge G, Atalay Pinar B, Altunbek Mine, Kalkan Batuhan M, Dogan Soner
a Department of Biophysics, School of Medicine , Yeditepe University , Istanbul , Turkey.
b Department of Medical Biology, School of Medicine , Maltepe University , Istanbul , Turkey.
Nutr Cancer. 2017 Oct;69(7):1003-1010. doi: 10.1080/01635581.2017.1359314. Epub 2017 Sep 18.
Adropin is a peptide hormone that has been implicated in insulin resistance and as a potential regulator of growth. The aim of this study is to determine the effect of calorie restriction on circulating levels of adropin in the MMTV-TGFα breast cancer mouse model and investigate the effects of adropin peptide on the viability of MCF-7 and MDA-231 breast cancer cells in culture. Ten-week-old mice were assigned to either ad libitum-fed (AL), chronic calorie-restricted, or intermittent calorie-restricted groups. Concentrations of serum adropin were measured using an enzyme-linked immunosorbent assay. Results showed an inverse correlation between serum adropin levels and mouse age that was attenuated by calorie restriction. In the AL group the level of adropin was significantly lower at week 50 compared to levels at week 10. However, among the calorie-restricted groups, serum levels of adropin remained high at week 50. The cell-line-specific effects were observed after treatment of cancer cell lines with a series of adropin concentrations (5, 10, 25, 50 ng/mL). Flow cytometry analysis showed that MCF-7 cells entered the early phase of apoptosis after treatment with 50 ng/mL for 24 h. Adropin may be involved in the protective effects that calorie restriction has on breast cancer risk.
内脂素是一种肽类激素，与胰岛素抵抗有关，并且可能是生长的调节因子。本研究的目的是确定热量限制对MMTV-TGFα乳腺癌小鼠模型中循环内脂素水平的影响，并研究内脂素肽对培养的MCF-7和MDA-231乳腺癌细胞活力的影响。将10周龄的小鼠分为自由进食（AL）组、慢性热量限制组或间歇性热量限制组。使用酶联免疫吸附测定法测量血清内脂素浓度。结果显示血清内脂素水平与小鼠年龄呈负相关，热量限制可减弱这种相关性。在AL组中，与第10周时的水平相比，第50周时内脂素水平显著降低。然而，在热量限制组中，第50周时血清内脂素水平仍然很高。在用一系列内脂素浓度（5、10、25、50 ng/mL）处理癌细胞系后，观察到了细胞系特异性效应。流式细胞术分析显示，用50 ng/mL处理24小时后，MCF-7细胞进入凋亡早期。内脂素可能参与了热量限制对乳腺癌风险的保护作用。
