Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 221 00 Lund, Sweden.
Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 221 00 Lund, Sweden.
J Colloid Interface Sci. 2018 Jan 1;509:463-471. doi: 10.1016/j.jcis.2017.09.046. Epub 2017 Sep 11.
Manipulation of specific binding and recycling of materials are two important aspects for practical applications of molecularly imprinted polymers. In this work, we developed a new approach to control the dynamic assembly of molecularly imprinted nanoparticles by surface functionalization. Molecularly imprinted polymer nanoparticles with a well-controlled core-shell structure were synthesized using precipitation polymerization. The specific binding sites were created in the core during the first step imprinting reaction. In the second polymerization step, epoxide groups were introduced into the particle shell to act asan intermediate linker to immobilize phenylboronic acids, as well as to introduce cis-diol structures on surface. The imprinted polymer nanoparticles modified with boronic acid and cis-diol structures maintained high molecular binding specificity, and the nanoparticles could be induced to form dynamic particle aggregation that responded to pH variation and chemical stimuli. The possibility of modulating molecular binding and nanoparticle assembly in a mutually independent fashion can be exploited in a number of applications where repeated use of precious nanoparticles is needed.
对材料的特异性结合和循环利用的调控是分子印迹聚合物实际应用中的两个重要方面。在这项工作中，我们通过表面功能化开发了一种控制分子印迹纳米粒子动态组装的新方法。采用沉淀聚合合成了具有良好控制的核壳结构的分子印迹聚合物纳米粒子。在第一步印迹反应中，在核内形成特异性结合位点。在第二步聚合反应中，将环氧化物基团引入到粒子壳中，作为中间连接体来固定苯硼酸，以及在表面引入顺式二醇结构。修饰有硼酸和顺式二醇结构的印迹聚合物纳米粒子保持了高的分子结合特异性，并且纳米粒子可以被诱导形成响应 pH 值变化和化学刺激的动态粒子聚集。在需要重复使用宝贵纳米粒子的许多应用中，可以利用这种相互独立地调节分子结合和纳米粒子组装的可能性。
