网格蛋白介导的内吞作用的机械调节。
Mechanoregulation of clathrin-mediated endocytosis.
机构信息
Department of Physics, Ohio State University, Columbus, OH 43210, USA.
Department of Biology, Capital University, Columbus, OH 43209, USA.
出版信息
J Cell Sci. 2017 Nov 1;130(21):3631-3636. doi: 10.1242/jcs.205930. Epub 2017 Sep 18.
Abstract
We characterized the tension response of clathrin-mediated endocytosis by using various cell manipulation methodologies. Elevated tension in a cell hinders clathrin-mediated endocytosis through inhibition of coat initiation, elongation of clathrin coat lifetimes and reduction of high-magnitude growth rates. Actin machinery supplies an inward pulling force necessary for internalization of clathrin coats under high tension. These findings suggest that the physical cues cells receive from their microenvironment are major determinants of clathrin-mediated endocytic activity.
摘要
我们使用各种细胞操作方法来描述网格蛋白介导的胞吞作用的张力反应。细胞内张力的增加通过抑制 衣被起始、延长网格蛋白衣被寿命和降低高幅度增长率来阻碍网格蛋白介导的胞吞作用。肌动蛋白机制为网格蛋白包被内化提供了必要的内向拉力，在高张力下进行内化。这些发现表明，细胞从其微环境中接收到的物理线索是网格蛋白介导的胞吞作用活性的主要决定因素。
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