Chen Weiqing, Ye Lijun, Wen Dengcheng, Chen Feihua
Department of General Surgery, The People's Hospital of Lin'an City, No 548 Yijing Street, Jincheng town, Lin'an City, Zhejiang Province, 311300, China.
Department of Gynecology and Obstetrics, The People's Hospital of Lin'an City, Lin'an City, Zhejiang Province, 311300, China.
Pathol Oncol Res. 2019 Jan;25(1):1-9. doi: 10.1007/s12253-017-0305-4. Epub 2017 Sep 19.
Studies have investigated the effect of ROBO1. All the same, the relationship between miR-490-5p and ROBO1, and the underlying mechanism are still unclear. We aimed to study the effect of microRNA-490-5p (miR-490-5p) on hepatocellular carcinoma (HCC) cell proliferation, migration and invasion by directly regulating ROBO1. The expression of miR-490-5p and ROBO1 in HCC tissues and cells were tested by RT-qPCR, and the Hep3B cells were selected for subsequent experiments. We confirmed the relationship between miR-490-5p and ROBO1 by luciferase reporter system. The effects of miR-490-5p on cell proliferation, migration and invasion of Hep3B cells were assessed by MTT assay, colony formation assay, wound healing assay and transwell assay, respectively. Flow cytometry was employed to detect the influence of miR-490-5p on cell cycle and apoptosis of Hep3B cells. The expression of miR-490-5p was down-regulated, while ROBO1 was up-regulated in HCC tissues and cells than the controls. MiR-490-5p can target ROBO1. MiR-490-5p inhibited cell proliferation, migration and invasion, but promoted cell apoptosis of Hep3B cells by inhibiting ROBO1. We confirmed that miR-490-5p could directly suppress ROBO1, which might be a potential mechanism in inhibiting HCC cell proliferation, migration and invasion.
已有研究对ROBO1的作用进行了调查。尽管如此，miR-490-5p与ROBO1之间的关系及其潜在机制仍不清楚。我们旨在研究微小RNA-490-5p（miR-490-5p）通过直接调控ROBO1对肝细胞癌（HCC）细胞增殖、迁移和侵袭的影响。采用RT-qPCR检测miR-490-5p和ROBO1在HCC组织和细胞中的表达，并选择Hep3B细胞进行后续实验。通过荧光素酶报告系统证实了miR-490-5p与ROBO1之间的关系。分别采用MTT法、集落形成法、伤口愈合试验和Transwell试验评估miR-490-5p对Hep3B细胞增殖、迁移和侵袭的影响。采用流式细胞术检测miR-490-5p对Hep3B细胞周期和凋亡的影响。与对照组相比，HCC组织和细胞中miR-490-5p表达下调，而ROBO1表达上调。miR-490-5p可靶向ROBO1。miR-490-5p通过抑制ROBO1抑制Hep3B细胞的增殖、迁移和侵袭，但促进细胞凋亡。我们证实miR-490-5p可直接抑制ROBO1，这可能是抑制HCC细胞增殖、迁移和侵袭的潜在机制。
