Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
Pest Manag Sci. 2018 Mar;74(3):557-568. doi: 10.1002/ps.4735. Epub 2017 Nov 13.
Use of MON 810 maize (Zea mays), which expresses the insecticidal protein Cry1Ab from Bacillus thuringiensis (Bt maize), is a highly effective method to control Sesamia nonagrioides (Lefèbvre), a key maize pest in Mediterranean countries. Monitoring programs to assess the potential development of resistance of target pests to Bt maize are mandatory in the European Union (EU). Here we report the results of the S. nonagrioides resistance monitoring plan implemented for MON 810 maize in the EU between 2004 and 2015 and reassess the different components of this long-term harmonized plan.
No major shifts in the susceptibility of S. nonagrioides to the Cry1Ab protein have occurred over time. The reassessment of this long-term program has identified some practical and technical constraints, allowing us to provide specific recommendations for improvement: use reference strains instead of susceptibility baselines as comparators for field-collected populations; shift from dose-response bioassays to diagnostic concentrations; and focus monitoring on areas with high adoption rates, such as the Ebro basin in Spain.
There are no signs of field resistance of S. nonagrioides to the Cry1Ab protein of MON 810 maize. Specific recommendations for improvement are provided, based on the knowledge and experience accumulated through the implementation of this unique EU-wide harmonized plan. © 2017 Society of Chemical Industry.
使用表达苏云金芽孢杆菌 Cry1Ab 杀虫蛋白的 MON810 玉米（玉米）是控制地中海国家玉米主要害虫烟夜蛾（Sesamia nonagrioides）的一种非常有效的方法。在欧盟（EU），监测计划以评估目标害虫对 Bt 玉米产生抗性的潜在发展是强制性的。本文报告了 2004 年至 2015 年期间在欧盟实施的 MON810 玉米烟夜蛾抗性监测计划的结果，并重新评估了该长期协调计划的不同组成部分。
烟夜蛾对 Cry1Ab 蛋白的敏感性随时间没有发生重大变化。对该长期计划的重新评估确定了一些实际和技术上的限制，使我们能够提供具体的改进建议：使用参考菌株而不是敏感性基线作为田间采集种群的对照；从剂量反应生物测定转向诊断浓度；并将监测重点放在采用率高的地区，如西班牙的埃布罗盆地。
烟夜蛾对 MON810 玉米的 Cry1Ab 蛋白没有田间抗性的迹象。根据实施这一独特的欧盟范围内协调计划所积累的知识和经验，提出了具体的改进建议。 © 2017 化学工业协会。
