Akbulut Ulaş Emre, Çebi Alper Han, Sağ Elif, İkbal Mevlit, Çakır Murat
Department of Pediatric Gastroenterology Hepatology and Nutrition, Kanuni Training and Research Hospital, Trabzon, Turkey.
Turk J Gastroenterol. 2017 Nov;28(6):471-475. doi: 10.5152/tjg.2017.17092. Epub 2017 Sep 19.
BACKGROUND/AIMS: This study aimed to investigate polymorphisms in the genes responsible for encoding cytokines interleukin-6 (IL-6) (-572G/C) (rs1800796) and IL-17 (-197A/G) (rs2275913) in patients with celiac disease (CD). We further aimed to investigate the relationship between CD symptoms and histopathological findings and the relationship between these polymorphisms.
We compared the results with those of healthy control subjects to establish whether any of the polymorphisms are involved in the susceptibility to CD. Eighty-four patients with CD and 83 healthy controls were enrolled in this study. Children with CD were divided into two groups depending on whether their symptoms were typical or atypical. The IL-6 (-572G/C) and IL-17 (-197A/G) polymorphisms were genotyped based on a polymerase chain reaction coupled with restriction fragment length polymorphism.
Significant differences for the IL-6 (-572G/C) polymorphism were observed between patients with CD and controls (p=0.018, odds ratio (OR): 5.47, 95% confidence interval (CI): 1.161-25.800). No statistically significant association was observed between the IL-17 (-197A/G) polymorphism and CD (p>0.05). In addition, the symptoms and histopathological findings of children with CD were not related to either of the polymorphisms.
The results of our study indicate that the IL-6 (-572G/C) polymorphism may play a role in susceptibility to CD.
背景/目的：本研究旨在调查乳糜泻（CD）患者中负责编码细胞因子白细胞介素-6（IL-6）（-572G/C）（rs1800796）和白细胞介素-17（IL-17）（-197A/G）（rs2275913）的基因多态性。我们还旨在研究CD症状与组织病理学结果之间的关系以及这些多态性之间的关系。
我们将结果与健康对照者的结果进行比较，以确定是否有任何多态性参与了CD的易感性。本研究纳入了84例CD患者和83名健康对照者。患有CD的儿童根据其症状是典型还是非典型分为两组。基于聚合酶链反应结合限制性片段长度多态性对IL-6（-572G/C）和IL-17（-197A/G）多态性进行基因分型。
在CD患者与对照者之间观察到IL-6（-572G/C）多态性存在显著差异（p = 0.018，优势比（OR）：5.47，95%置信区间（CI）：1.161 - 25.800）。未观察到IL-17（-197A/G）多态性与CD之间存在统计学显著关联（p>0.05）。此外，患有CD的儿童的症状和组织病理学结果与任何一种多态性均无关。
我们的研究结果表明，IL-6（-572G/C）多态性可能在CD易感性中起作用。
