Azab Seham F, Abdalhady Mohamed A, Elsaadany Hosam F, Elkomi Mohamed A, Elhindawy Eman M, Sarhan Dina T, Salam Mohamed M A, Allah Mayy A N, Emam Ahmed A, Noah Maha A, Abdelsalam Nasser I, Abdellatif Sawsan H, Rass Anwar A, Ismail Sanaa M, Gheith Tarek, Aziz Khalid A, Hamed Mohammed E, Abdelrahman Hind M, Ahmed Ahmed R, Nabil Rehab M, Abdulmaksoud Rehab S, Yousef Hala Y
Faculty of Medicine, Zagazig University, Egypt.
Medicine (Baltimore). 2016 Jun;95(26):e4013. doi: 10.1097/MD.0000000000004013.
Community-acquired pneumonia (CAP) is one of the leading causes of death worldwide. Cytokines are involved in the pathogenesis of CAP. To date, only a few studies concerned the association of interleukin-10 (IL-10) gene polymorphisms with CAP.In this study, we aimed to investigate whether the -1082(G/A) polymorphism in the promoter region of the IL-10 gene is involved in susceptibility to and the outcome of CAP, and we also measured the serum level of IL-10 to assess its relation to such polymorphism.This was a case-control study included 100 patients with CAP, and matched with age, gender, and ethnicity of 100 healthy control children. IL-10 -1082(G/A) gene polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism, while the serum IL-10 levels were measured by ELISA method.Compared to the controls subjects, the frequencies of the IL-10 -1082 AA genotype and A allele were observed to be overrepresented in patients with CAP (51%; odds ratio [OR] = 2.8; 95% confidence interval [CI]: 1.5-5.3 for the AA genotype; P < 0.01) and (70%; OR: 1.95; 95% CI: 1.27-3.00 for the A allele; P < 0.01, respectively). We found that patients with the GG genotype had significantly higher serum IL-10 levels (46.7 ± 9.5 pg/mL) compared to those with AG genotype (21.8 ± 4.5 pg/mL) and AA genotype (11.5 ± 3.3 pg/mL); P < 0.01, respectively. Our data revealed a significant positive association between the -1082 GG genotype and susceptibility to severe sepsis, acute respiratory failure, and hospital mortality (OR: 3.8; 95% CI: 1.3-11.2; P < 0.01).We demonstrate for the first time, to the best of our knowledge, that IL-10 -1082 (G/A) gene polymorphism may contribute to susceptibility to CAP in Egyptian children. Moreover, we observed that the presence of a G allele or GG genotype at the -1082 position of the promoter region of the IL-10 gene constitute risk factors for developing severe sepsis, acute respiratory failure, and hospital mortality among patients with CAP.
社区获得性肺炎（CAP）是全球主要的死亡原因之一。细胞因子参与CAP的发病机制。迄今为止，仅有少数研究关注白细胞介素-10（IL-10）基因多态性与CAP的关联。在本研究中，我们旨在调查IL-10基因启动子区域的-1082（G/A）多态性是否与CAP的易感性及预后相关，并且我们还检测了血清IL-10水平以评估其与这种多态性的关系。
这是一项病例对照研究，纳入了100例CAP患者，并与100名健康对照儿童按年龄、性别和种族进行匹配。通过聚合酶链反应-限制性片段长度多态性对IL-10 -1082（G/A）基因多态性进行基因分型，同时采用酶联免疫吸附测定法检测血清IL-10水平。
与对照受试者相比，观察到CAP患者中IL-10 -1082 AA基因型和A等位基因的频率过高（AA基因型为51%；优势比[OR]=2.8；95%置信区间[CI]：1.5 - 5.3；P<0.01）以及（A等位基因为70%；OR：1.95；95%CI：1.27 - 3.00；P<0.01）。我们发现，与AG基因型（21.8±4.5 pg/mL）和AA基因型（11.5±3.3 pg/mL）的患者相比，GG基因型的患者血清IL-10水平显著更高（46.7±9.5 pg/mL）；P<0.01。我们的数据显示-1082 GG基因型与严重脓毒症、急性呼吸衰竭及医院死亡率的易感性之间存在显著正相关（OR：3.8；95%CI：1.3 - 11.2；P<0.01）。
据我们所知，我们首次证明IL-10 -1082（G/A）基因多态性可能与埃及儿童CAP的易感性有关。此外，我们观察到IL-10基因启动子区域-1082位点存在G等位基因或GG基因型是CAP患者发生严重脓毒症、急性呼吸衰竭及医院死亡率的危险因素。
